Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

Sirtuin Activity Assay Kit (Fluorometric) Kit

AcA Fluorometric Purified Protein, Tissue Samples
Catalog No. ABIN2487095
$480.00
Plus shipping costs $45.00
100 tests
local_shipping Shipping to: United States
Delivery in 2 to 3 Business Days
  • Target
    Sirtuin
    Detection Method
    Fluorometric
    Application
    Activity Assay (AcA)
    Sample Type
    Purified Protein, Tissue Samples
    Characteristics
    Sirtuin Activity Assay Kit, the acetylated p53-AFC substrate is deacetylated by Sirtuins in the presence of NAD+ to generate the deacetylated p53-AFC substrate, nicotinamide and O-Acetyl-ADP Ribose. Cleavage of the deacetylated p53-AFC substrate by the Developer releases the fluorescent group, which is detected fluorometrically at Ex/Em = 400/505 nm. HDAC's also deacetylate the acetylated p53-AFC substrate. Trichostatin A is added to the reaction to specifically inhibit HDAC's in samples. This kit provides a rapid, simple, sensitive, and reliable test to measure Sirtuin Activity in a variety of samples. The limit of quantification of the assay is 0.06 μU of recombinant human SIRT6.
    Detection of Sirtuin Activity in variety of samples
    Components
    Sirtuin Assay Buffer
    Homogenization Buffer
    1M DTT
    Substrate (in DMSO)
    NAD
    Positive Control
    Trichostatin A (in DMSO)
    Developer
    AFC Standard (in DMSO) (1 mM)
  • Application Notes
    Detection of Sirtuin Activity in variety of samples
    Comment

    Further details regarding sample type:

    • Purified recombinant protein
    • Cell and tissue lysate
    • Nuclear Extract
    • Mitochondria
    • Immunoprecipitated samples

    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Expiry Date
    12 months
  • Target
    Sirtuin
    Synonyms
    SIRT4, sirtuin 5, sirtuin 4, SIRT5, SIRT4
    Background
    Sirtuins are a class of proteins that possess either histone deacetylase or mono-ribosyltransferase activity. Sirtuins are localized in the cytoplasm, nucleus, nucleolus as well as mitochondria. They are associated with aging, cellular protection, sugar metabolism and cell cycle regulation. Unlike other known protein deacetylases, which simply hydrolyze acetyl-lysine residues, the sirtuin-mediated deacetylation reaction hydrolyzes acetyl-lysine and NAD. This hydrolysis yields the deacetylated substrate, O-acetyl-ADP-ribose and nicotinamide, itself an inhibitor of sirtuin activity. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. In
You are here:
help Support