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Insulin ELISA Kit

INS Reactivity: Mouse Colorimetric Sandwich ELISA 15.6-1000 nlU/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Catalog No. ABIN2641860
  • Target See all Insulin (INS) ELISA Kits
    Insulin (INS)
    Reactivity
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    Mouse
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    15.6-1000 nlU/mL
    Minimum Detection Limit
    15.6 nlU/mL
    Application
    ELISA
    Purpose
    For the quantitative determination of mouse insulin (INS) concentrations in serum, plasma, cell culture supernates, tissue homogenates, cell lysates.
    Sample Type
    Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate, Cell Lysate
    Analytical Method
    Quantitative
    Specificity
    This assay has high sensitivity and excellent specificity for detection of mouse INS.
    Cross-Reactivity (Details)
    Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between the target antigen and all analogues for other species. Therefore, cross reaction may still exist.
    Sensitivity
    3.9 nlU/mL
    Components
    • Assay plate (12 × 8 coated Microwells)
    • Standard (freeze dried)
    • Biotin-antibody (100 × concentrate)
    • HRP-avidin (100 × concentrate)
    • Biotin-antibody Diluent
    • HRP-avidin Diluent
    • Sample Diluent
    • Wash Buffer (25 × concentrate)
    • TMB Substrate
    • Stop Solution
    • Adhesive Strip (for 96 wells)
    • Instruction manual
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  • Application Notes
    • The supplier is only responsible for the kit itself, but not for the samples consumed during the assay. The user should calculate the possible amount of the samples used in the whole test. Please reserve sufficient samples in advance.
    • Samples to be used within 5 days may be stored at 2-8°C, otherwise samples must be stored at -20°C (≤ 1 month) or -80°C (≤ 2 months) to avoid loss of bioactivity and contamination.
    • Grossly hemolyzed samples are not suitable for use in this assay.
    • If the samples are not indicated in the manual, a preliminary experiment to determine the validity of the kit is necessary.
    • Please predict the concentration before assaying. If values for these are not within the range of the standard curve, users must determine the optimal sample dilutions for their particular experiments.
    • Tissue or cell extraction samples prepared by chemical lysis buffer may cause unexpected ELISA results due to the impacts of certain chemicals.
    • Owing to the possibility of mismatching between antigens from another resource and antibodies used in this supplier's kits (e.g., antibody targets conformational epitope rather than linear epitope), some native or recombinant proteins from other manufacturers may not be recognized by this supplier's products.
    • Influenced by factors including cell viability, cell number and cell sampling time, samples from cell culture supernatant may not be recognized by the kit.
    • Fresh samples without long time storage are recommended for the test. Otherwise, protein degradation and denaturalization may occur in those samples and finally lead to wrong results.
    Comment

    Detection wavelength: 450 nm

    Information on standard material:
    Depending on the antigen to be detected, standards can be either native or recombinant protein. The recombinant proteins are being expressed in CHO cells in most cases. Please inquire for more information. The formulation of auxiliary material in the standard is considered proprietary information, however it does not contain any poisonous substance. Proclin 300 (1:3000) is used as preservative.

    Information on reagents:
    In most cases the stop solution provided is 1 N H2SO4. The formulation of wash solution is proprietary information. None of the components contain (sodium) azide, thimerosal, 2-mercaptoethanol (2-ME) or any other poisonous materials. For the sandwich method kits, the sample diluent, antibody diluent, enzyme diluent and standard all contain BSA.

    Information on antibodies:
    The antibodies provided in different kits vary in regards to clonality and host. Some antibodies are affinity purified, some are Protein A

    Sample Volume
    100 μL
    Assay Time
    1 - 4.5 h
    Plate
    Pre-coated
    Protocol
    This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for INS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any INS present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for INS is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of INS bound in the initial step. The color development is stopped and the intensity of the color is measured.
    Assay Precision
    Intra-assay precision (precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess precision.
    Inter-assay precision (precision between assays): Three samples of known concentration were tested in twenty assays to assess precision.
    • Intra-assay: CV% less than 8%
    • Inter-assay: CV% less than 10%
    Restrictions
    For Research Use only
  • Precaution of Use
    The Stop Solution provided with this kit is an acid solution. Wear eye, hand, face and clothing protection when using this material.
    Handling Advice
    • The kit should not be used beyond the expiration date on the kit label.
    • Do not mix or substitute reagents with those from other lots or sources.
    • If samples generate values higher than the highest standard, dilute the samples with Sample Diluent and repeat the assay.
    • Any variation in Sample Diluent, operator, pipetting technique, washing technique, incubation time/temperature and kit age can cause variation in binding.
    • This assay is designed to eliminate interference by soluble receptors, binding proteins and other factors present in biological samples. Until all factors have been tested in the Immunoassay, the possibility of interference cannot be excluded.
    Storage
    4 °C/-20 °C
    Storage Comment
    For unopened kit: All the reagents should be kept according to the labels on vials.
    Expiry Date
    6 months
  • Li, Zhou, Chen, Zhang, Wang: "Kukoamine A attenuates insulin resistance and fatty liver through downregulation of Srebp-1c." in: Biomedicine & pharmacotherapy, Vol. 89, pp. 536-543, (2018) (PubMed).

    Zeng, He, Jia, Hao: "Lycopene Improves Insulin Sensitivity through Inhibition of STAT3/Srebp-1c-Mediated Lipid Accumulation and Inflammation in Mice fed a High-Fat Diet." in: Experimental and clinical endocrinology & diabetes : official journal, German Society of Endocrinology [and] German Diabetes Association, Vol. 125, Issue 9, pp. 610-617, (2018) (PubMed).

    Zhuang, Shou, Lu, Wang, Qiu, Wang, He, Chen, Jiao, Zhang: "Arachidonic acid sex-dependently affects obesity through linking gut microbiota-driven inflammation to hypothalamus-adipose-liver axis." in: Biochimica et biophysica acta. Molecular basis of disease, Vol. 1863, Issue 11, pp. 2715-2726, (2018) (PubMed).

    Skurikhin, Pakhomova, Pershina, Ermolaeva, Krupin, Ermakova, Pan, Kudryashova, Rybalkina, Pavlovskaya, Litvyakov, Goldberg, Dygai: "Regenerative Potential of Spermatogonial Stem Cells, Endothelial Progenitor Cells, and Epithelial Progenitor Cells of C57Bl/6 Male Mice with Metabolic Disorders." in: Bulletin of experimental biology and medicine, Vol. 163, Issue 2, pp. 239-244, (2018) (PubMed).

    Li, Wang, Xu, Luo, Luo, Hao, Cheng, Fang, Wang, Zhang, Chen: "Berberine Improves Diabetic Encephalopathy Through the SIRT1/ER Stress Pathway in db/db Mice." in: Rejuvenation research, Vol. 21, Issue 3, pp. 200-209, (2018) (PubMed).

    Xu, Bai, Chen, Li, Qin, Meng, Zhang: "6-Shogaol ameliorates diabetic nephropathy through anti-inflammatory, hyperlipidemic, anti-oxidative activity in db/db mice." in: Biomedicine & pharmacotherapy, Vol. 97, pp. 633-641, (2018) (PubMed).

    Zhou, He, Zuo, Zhang, Wan, Long, Huang, Wu, Wu, Liu, Yin: "Serine prevented high-fat diet-induced oxidative stress by activating AMPK and epigenetically modulating the expression of glutathione synthesis-related genes." in: Biochimica et biophysica acta. Molecular basis of disease, Vol. 1864, Issue 2, pp. 488-498, (2018) (PubMed).

    Skurikhin, Pakhomova, Pershina, Krupin, Ermakova, Pan, Kudryashova, Ermolaeva, Khmelevskaya, Goldberg, Zhdanov, Dygai: "Role of Sertoli and Leydig Cells in the Regulation of Spermatogonial Stem Cell and Development of Reproductive Disorders in Male C57Bl/6 Mice with Type 1 Diabetes Mellitus." in: Bulletin of experimental biology and medicine, Vol. 164, Issue 2, pp. 127-131, (2018) (PubMed).

    Wang, Chen, Du, Long, Reed, Hu, Suppiramaniam, Hong, Tang: "Targeted inhibition of RAGE reduces amyloid-β influx across the blood-brain barrier and improves cognitive deficits in db/db mice." in: Neuropharmacology, Vol. 131, pp. 143-153, (2018) (PubMed).

    Tang, Wu, Lei, Yi, Xu, Lan, Yang, Liu: "HIF1α deletion facilitates adipose stem cells to repair renal fibrosis in diabetic mice." in: In vitro cellular & developmental biology. Animal, Vol. 54, Issue 4, pp. 272-286, (2018) (PubMed).

    Liu, Zhai, Guo, Shi, Liu, Wang, Zhang, Jing, Hai: "Whole Body Vibration Improves Insulin Resistance in db/db Mice: Amelioration of Lipid Accumulation and Oxidative Stress." in: Applied biochemistry and biotechnology, Vol. 179, Issue 5, pp. 819-29, (2017) (PubMed).

    Sun, Zhang, Zheng, Zhuang, Li, Lu, Quan, Dong, Zheng, Xiu: "Trivaric acid, a new inhibitor of PTP1b with potent beneficial effect on diabetes." in: Life sciences, Vol. 169, pp. 52-64, (2017) (PubMed).

    Shi, Xiong, Dai, Deng, Sun, Hu, Zhou, Xu: "Nuclear orphan receptor TLX affects gene expression, proliferation and cell apoptosis in beta cells." in: Biochemical and biophysical research communications, Vol. 468, Issue 1-2, pp. 387-93, (2016) (PubMed).

    Wang, Chen, Zhong, Tang, Hu, Long, Miao, Liao, Sun, Hong: "PPARγ agonists regulate bidirectional transport of amyloid-β across the blood-brain barrier and hippocampus plasticity in db/db mice." in: British journal of pharmacology, Vol. 173, Issue 2, pp. 372-85, (2016) (PubMed).

    Tong, Ma, Ge, Mo, Zhou, He, Wang: "Dicaffeoylquinic Acid-Enriched Fraction of Cichorium glandulosum Seeds Attenuates Experimental Type 1 Diabetes via Multipathway Protection." in: Journal of agricultural and food chemistry, Vol. 63, Issue 50, pp. 10791-802, (2016) (PubMed).

    Shi, Deng, Dai, Xu, Xiong, Ma, Cheng: "Nr2e1 Deficiency Augments Palmitate-Induced Oxidative Stress in Beta Cells." in: Oxidative medicine and cellular longevity, Vol. 2016, pp. 9648769, (2016) (PubMed).

    Zou, Yang, Xue, Ke, Huang, Huang, Dai, Sun, Xu: "WITHDRAWN: RIP140 mediates hyperglycemia-induced glucotoxicity in ?-cells via the activation of JNK and ERK1/2 signaling pathways." in: Diabetes research and clinical practice, (2015) (PubMed).

    Stockebrand, Hornig, Neu, Atzler, Cordts, Böger, Isbrandt, Schwedhelm, Choe: "Homoarginine supplementation improves blood glucose in diet-induced obese mice." in: Amino acids, (2015) (PubMed).

    Xie, Loi Truong, Zhang, Xu, Xu, Li: "Dan-Qi prescription ameliorates insulin resistance through overall corrective regulation of glucose and fat metabolism." in: Journal of ethnopharmacology, Vol. 172, pp. 70-9, (2015) (PubMed).

    Chen, Dong, Zhong, Ghosh, Tang, Long, Hu, Miao, Liao, Sun, Kong, Hong: "Antidiabetic drugs restore abnormal transport of amyloid-? across the blood-brain barrier and memory impairment in db/db mice." in: Neuropharmacology, Vol. 101, pp. 123-136, (2015) (PubMed).

  • Target See all Insulin (INS) ELISA Kits
    Insulin (INS)
    Abstract
    INS Products
    Synonyms
    IDDM2 ELISA Kit, ILPR ELISA Kit, IRDN ELISA Kit, MODY10 ELISA Kit, ins1 ELISA Kit, xins ELISA Kit, ins1-a ELISA Kit, Insulin ELISA Kit, AA986540 ELISA Kit, Ins-2 ELISA Kit, InsII ELISA Kit, Mody ELISA Kit, Mody4 ELISA Kit, proinsulin ELISA Kit, zgc:109842 ELISA Kit, igf2-A ELISA Kit, ins ELISA Kit, ins-a ELISA Kit, ins-b ELISA Kit, insulin ELISA Kit, insulin precursor ELISA Kit, insulin II ELISA Kit, preproinsulin ELISA Kit, insulin L homeolog ELISA Kit, insulin S homeolog ELISA Kit, INS ELISA Kit, INS-IGF2 ELISA Kit, ins ELISA Kit, Ins ELISA Kit, PIN ELISA Kit, Ins2 ELISA Kit, ins.L ELISA Kit, ins.S ELISA Kit
    Pathways
    NF-kappaB Signaling, RTK Signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation
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