AChR Ab ELISA Kit

Catalog No. ABIN2648477

Product Details AChR Ab ELISA Kit

Target: AChR Ab (Acetylcholine Receptor Antibody (AChR Ab))

Reactivity: Human

Detection Method: Colorimetric

Application: ELISA

Purpose: Anti-AChR (Acetylcholine Receptor) ELISA Assay kit is intended for the quantitative determination of Anti-AChR in serum by enzyme linked immunoassay (ELISA).

Sample Type: Serum

Analytical Method: Quantitative

Sensitivity: 0.23 nmol/l

Characteristics: The Anti-AChR ELISA Assay kit. Autoantibodies to the acetylcholine receptor (AChR) are responsible for failure of the neuromuscular junction in myasthenia gravis, a neuromuscular disease leading to fluctuating muscle weakness and fatigability. Measurement of these antibodies can be of considerable value in disease diagnosis and management.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Sample Volume: 100 μL

Protocol: The assay system depends on the ability of AChR Abs in human serum to bind to similar sites on the AChR as various monoclonal antibodies such as MAb1 (coated on ELISA plate wells) and/or MAb2 and/or MAb3 (which are labeled with biotin). In the absence of AChR Abs a complex is formed between MAb1 coated on the plate wells, the AChR and MAb2 and MAb3 biotin. MAb2 and MAb3 biotin bound are then detected by addition of streptavidin peroxidase (SA-POD), substrate (TMB) and stop solution. In the presence of AChR Abs the formation of the MAb1-AChR-MAb2/MAb3 biotin complex is inhibited, resulting in less SA-POD being bound and a reduction in final absorbance at 450nm. The higher the concentration of AChR Abs in the test serum the greater the inhibition of MAb biotin binding.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Target Details for AChR Ab

Target: AChR Ab (Acetylcholine Receptor Antibody (AChR Ab))

Alternative Name: Anti-AChR (Acetylcholine Receptor) (AChR Ab Products)

Target Type: Antibody, Antibody