Helicobacter Pylori Antigen ELISA Kit

Catalog No. ABIN2648675

Product Details

Target: Helicobacter Pylori Antigen

Reactivity: Helicobacter pylori

Detection Method: Colorimetric

Application: ELISA

Sample Type: Fecal

Sensitivity: 0.165 ng/ml

Characteristics: H. pylori (previously known as Campylobacter pyloridis) is a type of bacteria that infects the stomach and is a common cause of peptic ulcers. H. pylori bacteria can be passed from person to person through direct contact with saliva, vomit or fecal matter. H. pylori can also be spread through contaminated food or water.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Sample Volume: 100 μL

Assay Time: 2 h

Protocol: Assay calibrators and fecal specimen are added to microtiter wells of microplate that was coated with a highly purified monoclonal H. pylori antibody on its wall. During the assay, the H. pylori antigen will be bound to the antibody coated plate after an incubation period. The unbound material is washed away and another HRP-conjugated monoclonal antibody which specifically recognizes the protein of H. pylori is added for further immunoreactions. After an incubation period, the immunocomplex of "H. pylori Antibody - H. pylori Antigen - HRP-conjugated Anti-H. pylori Tracer Antibody" is formed if H. pylori antigen is present in the test sample. The unbound tracer antibody and other proteins in buffer matrix are removed in the subsequent washing step. HRP conjugated tracer antibody bound to the well is then incubated with a substrate solution in a timed reaction and then measured in a spectrophotometric microplate reader. The enzymatic activity of the tracer antibody bound to H. pylori proteins captured on the wall of each microtiter well is directly proportional to the amount of H. pylori antigen level in each test specimen.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Target Details

Target: Helicobacter Pylori Antigen

Alternative Name: H Pylori Antigen