IL-22 ELISA Kit

Catalog No. ABIN2648761

Product Details IL-22 ELISA Kit

Target: IL-22 (IL22) (Interleukin 22 (IL22))

Reactivity: Human

Detection Method: Colorimetric

Application: ELISA

Purpose: Human Interleukin 22 (IL-22) ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of human Interleukin 22 (IL-22) concentrations in cell culture supernates, serum, and plasma.

Sample Type: Serum, Plasma, Cell Culture Cells

Analytical Method: Quantitative

Sensitivity: 15 ng/ml

Characteristics: Human Interleukin 22 (IL-22) ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Sample Volume: 100 μL

Assay Time: 3.5 h

Plate: Pre-coated

Protocol: Human Interleukin 22 (IL-22) ELISA Assay Kit employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-22 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-22 present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for IL-22 is added to the wells and binds to the combination of capture antibody- IL-22 in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of IL-22 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-22 standard dilutions and IL-22 sample concentration determined.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Target Details for IL-22

Target: IL-22 (IL22) (Interleukin 22 (IL22))

Alternative Name: IL-22 (IL22 Products)

Synonyms: IL-21 ELISA Kit, IL-22 ELISA Kit, IL-D110 ELISA Kit, IL-TIF ELISA Kit, ILTIF ELISA Kit, TIFIL-23 ELISA Kit, TIFa ELISA Kit, zcyto18 ELISA Kit, IL-22a ELISA Kit, ILTIFa ELISA Kit, Iltif ELISA Kit, RGD1561292 ELISA Kit, interleukin-22 ELISA Kit, interleukin 22 ELISA Kit, IL22 ELISA Kit, il22 ELISA Kit, Il22 ELISA Kit