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2-ThioBarbituric Acid Reactive Substances (TBARS) ELISA Kit

Reactivity: Chemical Colorimetric Plasma, Serum, Urine
Catalog No. ABIN2648832
  • Target
    2-ThioBarbituric Acid Reactive Substances (TBARS)
    Reactivity
    Chemical
    Detection Method
    Colorimetric
    Application
    ELISA
    Sample Type
    Serum, Plasma, Urine
    Sensitivity
    0.5 μM
    Characteristics
    2-ThioBarbituric Acid Reactive Substances (TBARS) are naturally present in biological specimens and include lipid hydroperoxides and aldehydes which increase in concentration as a response to oxidative stress.1,2 TBARS assay values are usually reported in malonaldehyde (malondialdehyde, MDA) equivalents, a compound that results from the decomposition of polyunsaturated fatty acid lipid peroxides. The TBARS assay is a well-recognized, established method for quantifying these lipid peroxides, although it has been criticized for its reactivity towards other compounds other than MDA.3 This TBARS Cuvette Assay Kit offers the researcher a straightforward, reproducible and consistent method for analyzing urine for lipid peroxidation products.
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Sample Volume
    100 μL
    Assay Time
    0.5 h
    Plate
    Pre-coated
    Protocol
    This TBARS Cuvette Assay Kit is based on the reaction of a chromogenic reagent, 2-thiobarbituric acid, with MDA at 25 °C. One molecule of MDA reacts with 2 Molecules of 2-thiobarbituric acid via a Knoevenagel-type condensation to yield a chromophore with absorbance maximum at 532 nm,
    Restrictions
    For Research Use only
  • Storage
    4 °C
  • Target
    2-ThioBarbituric Acid Reactive Substances (TBARS)
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