Php Protein ELISA Kit

Details for Product No. ABIN2669298, Supplier: Log in to see
Antigen
Reactivity
Pasteurella piscicida (Photobacterium damsela subsp. piscicida)
Method Type
Competition ELISA
Application
ELISA
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Purpose Enzyme linked immunosorbent assay (ELISA) for the qualitative determination of anti-Php presence in Fish serum as an aid in the diagnosis of antibody production in Fish infected by Photobacterium damselae subsp. Piscicida. (...more...)
Sample Type Serum
Analytical Method Quantitative
Detection Method Colorimetric
Components 96 well polystyrene microplate ( 12 strip of 8 well ) coated with Photobacterium damselae subspecies piscicida inactivated bacteria and blocked with BSA 1% Plate Cover : 1 adhesive plate sealers Assay diluent: (Buffer A) 50 mL ready to use, with preservative Wash buffer concentrate: (Buffer B) 125 mL 10x to dilute to 1250 final volume with distilled water, with preservative. Conjugated Antibody: Rabbit Anti Fish Igs-HRP conjugated 20μl Color buffer: (Buffer C) 13 mL with preservative ABTS tablet: 1 Buffer C1: 13μl
Material not included Microplate reader capable of measuring absorbance at 415 nm Thermomixer, shaking water bath or rocking platform at 37 °C Precision pipettes and pipette tips Glass or plastic pipettes Deionized or distilled water Multi-channel pipette, semi-automated or automated microplate washer 1000 mL graduated cylinder for preparation of 1X Wash Buffer Vortex mixer Glass tubes
Application Notes Optimal working dilution should be determined by the investigator.
Comment

Sample or standards are pipetted directly into wells of a microplate pre-coated with Php inactivated bacteria and pre-blocked , that can capture anti- Igs thereby immobilizing to the well.

Plate Pre-coated
Restrictions For Research Use only
Precaution of Use The buffer C1 provided with this kit is H2O2 solution 30% m/m (110 volumes) that causes burns
Storage 4 °C
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