TNF alpha ELISA Kit

Catalog No. ABIN2690578

Product Details TNF alpha ELISA Kit

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Brand: BD OptEIA™

Analytical Method: Quantitative

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

BD OptEIA™ Human TNF ELISA Kit II

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: 1. Store unopened kit at 2 - 8°C. Do not use kit after expiration date.
2. Before use, bring all reagents to room temperature (18 - 25°C). Immediately after use, return to proper storage conditions.
3. Lyophilized standards are stable until kit expiration date. After reconstitution, use freshly reconstituted standard within 12 hours (stored at 2 - 8°C).

References for TNF alpha ELISA Kit (ABIN2690578)

Kidd, Emery: "The use of baculoviruses as expression vectors." in: Applied biochemistry and biotechnology, Vol. 42, Issue 2-3, pp. 137-59, (1993) (PubMed).

Ruddle: "Tumor necrosis factor (TNF-alpha) and lymphotoxin (TNF-beta)." in: Current opinion in immunology, Vol. 4, Issue 3, pp. 327-32, (1992) (PubMed).

Jäättelä: "Biologic activities and mechanisms of action of tumor necrosis factor-alpha/cachectin." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 64, Issue 6, pp. 724-42, (1991) (PubMed).

Vilcek, Lee: "Tumor necrosis factor. New insights into the molecular mechanisms of its multiple actions." in: The Journal of biological chemistry, Vol. 266, Issue 12, pp. 7313-6, (1991) (PubMed).

Beutler, Greenwald, Hulmes, Chang, Pan, Mathison, Ulevitch, Cerami: "Identity of tumour necrosis factor and the macrophage-secreted factor cachectin." in: Nature, Vol. 316, Issue 6028, pp. 552-4, (1985) (PubMed).

Wang, Creasey, Ladner, Lin, Strickler, Van Arsdell, Yamamoto, Mark: "Molecular cloning of the complementary DNA for human tumor necrosis factor." in: Science (New York, N.Y.), Vol. 228, Issue 4696, pp. 149-54, (1985) (PubMed).

Target Details for TNF alpha

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Alternative Name: TNF (TNF alpha Products)

Synonyms: DIF ELISA Kit, TNF-alpha ELISA Kit, TNFA ELISA Kit, TNFSF2 ELISA Kit, RATTNF ELISA Kit, Tnfa ELISA Kit, tnf ELISA Kit, TNF-a ELISA Kit, TNFalpha ELISA Kit, Tnfsf1a ELISA Kit, TNFa ELISA Kit, cTNF ELISA Kit, Tnf-alpha ELISA Kit, tnfa-like ELISA Kit, TNF-ALPHA ELISA Kit, dif ELISA Kit, tnfa ELISA Kit, xtnf ELISA Kit, tnfsf2 ELISA Kit, tnf-alpha ELISA Kit, Cachectin ELISA Kit, tumor necrosis factor ELISA Kit, tumor necrosis factor b (TNF superfamily, member 2) ELISA Kit, tumor necrosis factor alpha ELISA Kit, tumor necrosis factor a (TNF superfamily, member 2) ELISA Kit, TNF ELISA Kit, Tnf ELISA Kit, tnf ELISA Kit, tnfb ELISA Kit, tnf-alpha ELISA Kit, LOC103694380 ELISA Kit, tnfa ELISA Kit

Background: Tumor necrosis factor (TNF, formerly known as TNF-α), also known as cachectin, is a potent lymphoid factor which exerts cytotoxic effects on a wide range of tumor cells and certain other target cells. This cytokine is a primary regulator of inflammatory responses. TNF is produced by macrophages, neutrophils, activated T and B lymphocytes, endothelial cells, smooth muscle cells, astrocytes, natural killer cells, lymphokineactivated killer cells, and some transformed cells.
TNF, a 17.5 kD polypeptide of 157 amino acid residues, and LT-α (lymphotoxin alpha formerly known as TNF-α) are closely related (34 % a.a. homology), bind to the same cellular receptors (CD120a and CD120b), and produce similar effects. TNFs play a critical role in the body's resistance to infection by inducing fever and activating macrophages and in the destruction of certain tumors.
TNFs and Interleukin-1 function similarly. However, over-production of TNF has been associated with cytotoxic effects such as cachexia.
The BD OptEIA™ ELISA Kit II format was developed for superior accuracy with serum and plasma specimens. The data that demonstrates this enhancement can be located in the Performance Characteristics "Recovery" and "Linearity" sections.
The BD OptEIA Human TNF ELISA Kit II is for the quantitative determination of human TNF in serum, plasma, and cell culture supernatant.

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome