IL-1 beta ELISA Kit

Catalog No. ABIN2690985

Product Details IL-1 beta ELISA Kit

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Brand: BD OptEIA™

Analytical Method: Quantitative

Characteristics: The BD OptEIA™ Set contains the components necessary to develop enzyme - linked immunosorbent assays (ELISA). Sufficient materials are provided to yield approximately 20 plates of 96 wells if the recommended storage, materials, buffer preparation, and assay procedure are followed.

Components: Capture Antibody
Detection Antibody
Enzyme Reagent
Standards
Certificate of Analysis

Material not included: 1. 96-well Nunc-Immuno™ polystyrene Maxisorp ELISA flat bottom plates (ThermoFisher Scientific Cat. No. 442404) are recommended
2. Microplate reader capable of measuring absorbance at 450 nm
3. Precision pipettes
4. Graduated cylinder, one liter
5. Deionized or distilled water
6. Wash bottle or automated washer
7. Log-log graph paper or automated data reduction
8. Tubes to prepare standard dilutions
9. Laboratory timer
10. Plate sealers or parafilm

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

BD OptEIA™ Human IL-1β ELISA Set II - Reactivity Hu

Sample Volume: 100 µL

Plate: Without plate

Protocol: 1. Add 100 µL diluted Capture Ab to each well. Incubate overnight at 4°C.
2. Aspirate and wash 3 times.
3. Block plates: 200 µL Assay Diluent to each well. Incubate 1 hr RT
4. Aspirate and wash 3 times.
5. Add 100 µL standard or sample to each well. Incubate 2 hr RT.
6. Aspirate and wash 5 times.
7. Add 100 µL Working Detector (Detection Ab + SAv-HRP) to each well. Incubate 1 hr RT
8. Aspirate and wash 7 times (with 30 sec to 1 min soaks)
9. Add 100 µL Substrate Solution to each well. Incubate 30 min RT in dark
10. Add 50 µL Stop Solution to each well. Read at 450 nm within 30 min with l correction 570 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store unopened reagents at 2-8°C. Do not use reagents after expiration date, or if turbidity is evident.

Target Details for IL-1 beta

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Alternative Name: IL-1beta (IL1B Products)

Synonyms: IL-1 ELISA Kit, IL1-BETA ELISA Kit, IL1F2 ELISA Kit, IL-1BETA ELISA Kit, IL1beta ELISA Kit, il1-b ELISA Kit, zgc:111873 ELISA Kit, IL-1B ELISA Kit, IL-1beta ELISA Kit, Il-1b ELISA Kit, IL1B ELISA Kit, IL-1 beta ELISA Kit, IL-1b ELISA Kit, interleukin 1 beta ELISA Kit, interleukin 1, beta ELISA Kit, IL1B ELISA Kit, il1b ELISA Kit, Il1b ELISA Kit

Pathways: NF-kappaB Signaling, Interferon-gamma Pathway, TLR Signaling, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy, Cancer Immune Checkpoints, Inflammasome