ADAMTS1 ELISA Kit

Catalog No. ABIN2702804

Product Details ADAMTS1 ELISA Kit

Target: ADAMTS1 (ADAM Metallopeptidase with Thrombospondin Type 1 Motif, 1 (ADAMTS1))

Reactivity: Human, Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.4-100 ng/mL

Minimum Detection Limit: 0.4 ng/mL

Application: ELISA

Purpose: Mouse ADAMTS-1 (METH1) ELISA Kit for cell culture supernatants, plasma, and serum samples.

Sample Type: Cell Culture Supernatant, Plasma, Serum

Analytical Method: Quantitative

Specificity: This ELISA antibody pair detects mouse and human ADAMTS1. Other species not determined.

Sensitivity: 0.4 ng/mL

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Recommended Dilution for serum and plasma samples2 fold

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for ADAMTS1

Target: ADAMTS1 (ADAM Metallopeptidase with Thrombospondin Type 1 Motif, 1 (ADAMTS1))

Alternative Name: ADAMTS-1 (ADAMTS1 Products)

Synonyms: c3-c5 ELISA Kit, meth1 ELISA Kit, xadamts1 ELISA Kit, C3-C5 ELISA Kit, METH1 ELISA Kit, ADAM-TS1 ELISA Kit, ADAMTS ELISA Kit, ADAMTS-1 ELISA Kit, METH-1 ELISA Kit, adamts1 ELISA Kit, ADAM metallopeptidase with thrombospondin type 1 motif 1 ELISA Kit, ADAM metallopeptidase with thrombospondin type 1 motif, 1 ELISA Kit, a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 1 ELISA Kit, ADAM metallopeptidase with thrombospondin type 1 motif 1 L homeolog ELISA Kit, ADAMTS1 ELISA Kit, adamts1 ELISA Kit, Adamts1 ELISA Kit, adamts1.L ELISA Kit

Background: Gene Names: Adamts1
Protein names: A disintegrin and metalloproteinase with thrombospondin motifs 1 (ADAM-TS 1) (ADAM-TS1) (ADAMTS-1) (EC 3.4.24.-)

Gene ID: 11504

UniProt: P97857

Pathways: SARS-CoV-2 Protein Interactome