APOC3 ELISA Kit

Catalog No. ABIN2702832

Product Details APOC3 ELISA Kit

Target: APOC3 (Apolipoprotein C-III (APOC3))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 2.5-10.000 pg/mL

Minimum Detection Limit: 2.5 pg/mL

Application: ELISA

Purpose: Human ApoC3 ELISA Kit for cell culture supernatants, plasma, and serum samples.

Sample Type: Cell Culture Supernatant, Plasma, Serum

Analytical Method: Quantitative

Specificity: Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human Alpha-2 Microglobulin, ApoA, ApoC1, ApoC2, ApoE, ApoH, Artemin, BMP-6, Calbindin, CK beta 8-1 (CCL23), CNTF, EMMPRIN (CD147), Endostatin, Fibrinogen, FLRG, TNFSF18 (GITR Ligand), GPBB, Hemoglobin, HTRA2, IL-17E, IL-1 R3 (IL1 R Acp), Lactoferrin, Mesothelin, PAPP-A, PD-ECGF (Thymidine Phosphorylase), PDGF R alpha, Pepsinogen 2, Periostin, Persephin, PTH, RAP, SAA (Serum Amyloid A), Serpin F1 (PEDF), Tie-1, TPA, Thrombospondin 2, Ubiquitin+1, Vitronectin, VWF

Sensitivity: 2.5 pg/mL

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Recommended Dilution for serum and plasma samples500,000 fold

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Sample Preparation:

For example, add 2 μL of serum/plasma into a tube with 998.0 μL Assay Diluent to prepare a 500-fold diluted sample. Mix thoroughly and then pipette 2 μL of prepared 500-fold diluted sample into a tube with 1998 μL Assay Diluent to prepare a final 500,000 fold diluted sample.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for APOC3

Target: APOC3 (Apolipoprotein C-III (APOC3))

Alternative Name: ApoC3 (APOC3 Products)

Synonyms: APOC3 ELISA Kit, APOCIII ELISA Kit, HALP2 ELISA Kit, Apoc ELISA Kit, ApoC-III ELISA Kit, apo-CIII ELISA Kit, Apo-CIII ELISA Kit, apolipoprotein C3 ELISA Kit, apolipoprotein C-III ELISA Kit, APOC3 ELISA Kit, Apoc3 ELISA Kit

Background: Gene Names: APOC3
Protein names: Apolipoprotein C-III (Apo-CIII) (ApoC-III) (Apolipoprotein C3)

Gene ID: 345

UniProt: P02656

Pathways: Carbohydrate Homeostasis, Lipid Metabolism