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BACE1 ELISA Kit

BACE1 Reactivity: Human, Mouse Colorimetric Sandwich ELISA 1.0-250 ng/mL Cell Culture Supernatant, Plasma, Serum
Catalog No. ABIN2702843
  • Target See all BACE1 ELISA Kits
    BACE1 (Beta-secretase 1 (BACE1))
    Reactivity
    • 5
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Mouse
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    1.0-250 ng/mL
    Minimum Detection Limit
    1.0 ng/mL
    Application
    ELISA
    Purpose
    Human BACE-1 ELISA Kit for cell culture supernatants, plasma, and serum samples.
    Sample Type
    Cell Culture Supernatant, Plasma, Serum
    Analytical Method
    Quantitative
    Specificity
    This ELISA antibody pair detects human BACE-1, it also can recognize mouse BACE-1.
    Sensitivity
    1.0 ng/mL
    Characteristics
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Material not included
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Application Notes
    Recommended Dilution for serum and plasma samples2 fold
    Sample Volume
    100 μL
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Expiry Date
    6 months
  • Target See all BACE1 ELISA Kits
    BACE1 (Beta-secretase 1 (BACE1))
    Alternative Name
    BACE-1 (BACE1 Products)
    Synonyms
    ASP2 ELISA Kit, BACE ELISA Kit, HSPC104 ELISA Kit, C76936 ELISA Kit, Bace ELISA Kit, MGC145931 ELISA Kit, BACE1 ELISA Kit, zgc:77409 ELISA Kit, beta-secretase 1 ELISA Kit, beta-site APP cleaving enzyme 1 ELISA Kit, beta-site APP-cleaving enzyme 1 ELISA Kit, BACE1 ELISA Kit, Bace1 ELISA Kit, bace1 ELISA Kit
    Background
    Gene Names: BACE1 BACE KIAA1149
    Protein names: Beta-secretase 1 (EC 3.4.23.46) (Aspartyl protease 2) (ASP2) (Asp 2) (Beta-site amyloid precursor protein cleaving enzyme 1) (Beta-site APP cleaving enzyme 1) (Memapsin-2) (Membrane-associated aspartic protease 2)
    Gene ID
    23621
    UniProt
    P56817
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