CD93 ELISA Kit

Catalog No. ABIN2702876

Product Details CD93 ELISA Kit

Target: CD93

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 24-6.000 pg/mL

Minimum Detection Limit: 24 pg/mL

Application: ELISA

Purpose: Human C1q R1 (CD93) ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.

Sample Type: Cell Culture Supernatant, Plasma, Serum

Analytical Method: Quantitative

Specificity: The antibody pair provided in this kit recognizes Human Complement Component C1q Receptor (CD93)

Sensitivity: 24 pg/mL

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Recommended Dilution for serum and plasma samples600 fold

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for CD93

Target: CD93

Alternative Name: C1qR1 (CD93 Products)

Synonyms: 6030404G09Rik ELISA Kit, AA145088 ELISA Kit, AA4.1 ELISA Kit, AW555904 ELISA Kit, C1qr1 ELISA Kit, C1qrp ELISA Kit, Ly68 ELISA Kit, C1QR1 ELISA Kit, C1qR(P) ELISA Kit, C1qRP ELISA Kit, CDw93 ELISA Kit, ECSM3 ELISA Kit, MXRA4 ELISA Kit, dJ737E23.1 ELISA Kit, C1qRp ELISA Kit, CD93 antigen ELISA Kit, CD93 molecule ELISA Kit, Cd93 ELISA Kit, CD93 ELISA Kit

Background: Gene Names: CD93 C1QR1 MXRA4
Protein names: Complement component C1q receptor (C1q/MBL/SPA receptor) (C1qR) (C1qR(p)) (C1qRp) (CDw93) (Complement component 1 q subcomponent receptor 1) (Matrix-remodeling-associated protein 4) (CD antigen CD93)

Gene ID: 22918

UniProt: Q9NPY3