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DAND5 ELISA Kit

DAND5 Reactivity: Human Colorimetric Sandwich ELISA 0.2-50 ng/mL Cell Culture Supernatant, Plasma, Serum
Catalog No. ABIN2702924
  • Target See all DAND5 ELISA Kits
    DAND5 (DAN Domain Family, Member 5 (DAND5))
    Reactivity
    • 2
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    0.2-50 ng/mL
    Minimum Detection Limit
    0.2 ng/mL
    Application
    ELISA
    Purpose
    Human COCO ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.
    Sample Type
    Cell Culture Supernatant, Plasma, Serum
    Analytical Method
    Quantitative
    Specificity
    The antibody pair provided in this kit recognizes human COCO.
    Sensitivity
    0.2 ng/mL
    Characteristics
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Material not included
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Application Notes
    Recommended Dilution for serum and plasma samples2 fold
    Sample Volume
    100 μL
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Expiry Date
    6 months
  • Target See all DAND5 ELISA Kits
    DAND5 (DAN Domain Family, Member 5 (DAND5))
    Alternative Name
    COCO (DAND5 Products)
    Synonyms
    CER2 ELISA Kit, CERL2 ELISA Kit, CKTSF1B3 ELISA Kit, COCO ELISA Kit, CRL2 ELISA Kit, DANTE ELISA Kit, GREM3 ELISA Kit, SP1 ELISA Kit, 51-B6 ELISA Kit, cer2 ELISA Kit, cerl2 ELISA Kit, cktsf1b3 ELISA Kit, coco ELISA Kit, crl2 ELISA Kit, dante ELISA Kit, grem3 ELISA Kit, xCoco ELISA Kit, Cerl-2 ELISA Kit, Cerr2 ELISA Kit, Dte ELISA Kit, DAN domain BMP antagonist family member 5 ELISA Kit, DAN domain family member 5, BMP antagonist S homeolog ELISA Kit, DAN domain family member 5, BMP antagonist ELISA Kit, DAND5 ELISA Kit, dand5.S ELISA Kit, Dand5 ELISA Kit
    Background
    Gene Names: DAND5 CER2 CKTSF1B3 GREM3 SP1
    Protein names: DAN domain family member 5 (Cerberus-like protein 2) (Cerl-2) (Cysteine knot superfamily 1, BMP antagonist 3) (Gremlin-3)
    Gene ID
    199699
    UniProt
    Q8N907
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