Endothelin 1 ELISA Kit

Catalog No. ABIN2702980

Product Details Endothelin 1 ELISA Kit

Target: Endothelin 1 (EDN1)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 10-1000 pg/mL

Minimum Detection Limit: 10 pg/mL

Application: ELISA

Purpose: Human Endothelin-1 ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.

Sample Type: Cell Culture Supernatant, Plasma, Serum

Analytical Method: Quantitative

Specificity: The antibody pair provided in this kit recognizes Human Endothelin-1 (EDN1)

Sensitivity: 10 pg/mL

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Recommended Dilution for serum and plasma samples2 fold

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

References for Endothelin 1 ELISA Kit (ABIN2702980)

Pahari, Gu, van Oeveren, El-Essawi, Harringer, Brouwer: "Effect of minimized perfusion circuit on brain injury markers carnosinase and brain-type fatty binding protein in coronary artery bypass grafting patients." in: Artificial organs, Vol. 37, Issue 2, pp. 128-35, (2013) (PubMed).

Said, Smith, Sanchez-Carbayo, Theodorescu: "Tumor endothelin-1 enhances metastatic colonization of the lung in mouse xenograft models of bladder cancer." in: The Journal of clinical investigation, Vol. 121, Issue 1, pp. 132-47, (2011) (PubMed).

Target Details for Endothelin 1

Target: Endothelin 1 (EDN1)

Alternative Name: Endothelin-1 (EDN1 Products)

Synonyms: et1 ELISA Kit, et-1 ELISA Kit, Edn1 ELISA Kit, EDN ELISA Kit, ET-1 ELISA Kit, ET1 ELISA Kit, PPET1 ELISA Kit, HDLCQ7 ELISA Kit, preproET ELISA Kit, Et1 ELISA Kit, fb14d01 ELISA Kit, wu:fb14d01 ELISA Kit, PPET-1 ELISA Kit, EDN1 ELISA Kit, edn1 ELISA Kit, edn1-a ELISA Kit, edn1-b ELISA Kit, endothelin 1 ELISA Kit, endothelin 1 S homeolog ELISA Kit, endothelin 1 L homeolog ELISA Kit, edn1 ELISA Kit, EDN1 ELISA Kit, Edn1 ELISA Kit, edn1.S ELISA Kit, edn1.L ELISA Kit

Background: Gene Names: EDN1
Protein names: Endothelin-1 (Preproendothelin-1) (PPET1) [Cleaved into: Endothelin-1 (ET-1), Big endothelin-1]

Gene ID: 1906

UniProt: P05305

Pathways: Hormone Transport, Negative Regulation of Hormone Secretion, Regulation of Systemic Arterial Blood Pressure by Hormones, cAMP Metabolic Process, Regulation of Muscle Cell Differentiation, Regulation of G-Protein Coupled Receptor Protein Signaling, Regulation of Cell Size