FABP2 ELISA Kit

Catalog No. ABIN2702992

Product Details FABP2 ELISA Kit

Target: FABP2 (Fatty Acid Binding Protein 2, Intestinal (FABP2))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 25 pg/mL-100 ng/mL

Minimum Detection Limit: 25 pg/mL

Application: ELISA

Purpose: Human FABP2 ELISA Kit for cell culture supernatants, plasma, and serum samples.

Sample Type: Plasma, Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificity: In this ELISA antibody pair format, no cross-reactivity was observed with rhFABP-1, 3, 5, 6, 7, 8 and 9.

Sensitivity: 25 pg/mL

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Recommended Dilution for serum and plasma samples2 fold

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

References for FABP2 ELISA Kit (ABIN2702992)

Ji, Xie, Yin, Qi, Chen, Bai, Wang, Zhao, Jiang, Jiang: "Bile acid receptor TGR5 overexpression is associated with decreased intestinal mucosal injury and epithelial cell proliferation in obstructive jaundice." in: Translational research : the journal of laboratory and clinical medicine, Vol. 182, pp. 88-102, (2017) (PubMed).

Target Details for FABP2

Target: FABP2 (Fatty Acid Binding Protein 2, Intestinal (FABP2))

Alternative Name: FABP2 (FABP2 Products)

Synonyms: I-FABP ELISA Kit, ifabp ELISA Kit, zgc:92264 ELISA Kit, IFABP ELISA Kit, fabpi ELISA Kit, i-fabp ELISA Kit, fabp2-A ELISA Kit, FABP2 ELISA Kit, FABPI ELISA Kit, FABP ELISA Kit, Fabpi ELISA Kit, fabp2 ELISA Kit, fabp2a ELISA Kit, fatty acid binding protein 2, intestinal ELISA Kit, fatty acid binding protein 2 ELISA Kit, fatty acid binding protein 2 L homeolog ELISA Kit, fabp2 ELISA Kit, FABP2 ELISA Kit, Fabp2 ELISA Kit, fabp2.L ELISA Kit

Background: Gene Names: FABP2 FABPI
Protein names: Fatty acid-binding protein, intestinal (Fatty acid-binding protein 2) (Intestinal-type fatty acid-binding protein) (I-FABP)

Gene ID: 2169

UniProt: P12104