Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

FGF12 ELISA Kit

FGF12 Reactivity: Human Colorimetric Sandwich ELISA 32-8.000 pg/mL Cell Culture Supernatant, Plasma, Serum
Catalog No. ABIN2703006
  • Target See all FGF12 ELISA Kits
    FGF12 (Fibroblast Growth Factor 12 (FGF12))
    Reactivity
    • 2
    • 2
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    32-8.000 pg/mL
    Minimum Detection Limit
    32 pg/mL
    Application
    ELISA
    Purpose
    Human FGF-12 ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.
    Sample Type
    Cell Culture Supernatant, Plasma, Serum
    Analytical Method
    Quantitative
    Specificity
    This ELISA antibody pair recognizes human FGF-12. Other species not yet determined.
    Sensitivity
    32 pg/mL
    Characteristics
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Material not included
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Featured
    Discover our best selling FGF12 ELISA Kit
    Top Product
    Discover our top product FGF12 ELISA Kit
  • Application Notes
    Recommended Dilution for serum and plasma samples2 fold
    Sample Volume
    100 μL
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Expiry Date
    6 months
  • Target See all FGF12 ELISA Kits
    FGF12 (Fibroblast Growth Factor 12 (FGF12))
    Alternative Name
    FGF-12 (FGF12 Products)
    Synonyms
    FHF1 ELISA Kit, FHF-1 ELISA Kit, MGC84150 ELISA Kit, FGF12 ELISA Kit, fgf12 ELISA Kit, zgc:153104 ELISA Kit, fhf1 ELISA Kit, fgf12b ELISA Kit, DKFZp459P139 ELISA Kit, FGF12B ELISA Kit, AV114868 ELISA Kit, FGF-12 ELISA Kit, Fhf1 ELISA Kit, Fhf1b ELISA Kit, fibroblast growth factor 12 ELISA Kit, fibroblast growth factor 12 S homeolog ELISA Kit, fibroblast growth factor 12a ELISA Kit, FGF12 ELISA Kit, fgf12.S ELISA Kit, fgf12a ELISA Kit, fgf12 ELISA Kit, Fgf12 ELISA Kit
    Background
    Gene Names: FGF12 FGF12B FHF1
    Protein names: Fibroblast growth factor 12 (FGF-12) (Fibroblast growth factor homologous factor 1) (FHF-1) (Myocyte-activating factor)
    Gene ID
    2257
    UniProt
    P61328
    Pathways
    Negative Regulation of Transporter Activity
You are here:
Support