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GPC1 ELISA Kit

GPC1 Reactivity: Human Colorimetric Sandwich ELISA 0.15-40 ng/mL Cell Culture Supernatant, Plasma, Serum
Catalog No. ABIN2703059
  • Target See all GPC1 ELISA Kits
    GPC1 (Glypican 1 (GPC1))
    Reactivity
    • 5
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    0.15-40 ng/mL
    Minimum Detection Limit
    0.15 ng/mL
    Application
    ELISA
    Purpose
    Human Glypican 1 ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.
    Sample Type
    Cell Culture Supernatant, Plasma, Serum
    Analytical Method
    Quantitative
    Specificity
    The antibody pair provided in this kit recognizes Human Glypican-1 (GPC1)
    Sensitivity
    0.15 ng/mL
    Characteristics
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Material not included
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Application Notes
    Recommended Dilution for serum and plasma samples3 fold
    Sample Volume
    100 μL
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Expiry Date
    6 months
  • Monteforte, Lam, Sherman, Henderson, Sligar, Spencer, Tang, Dunn, Baker: "* Glioblastoma Exosomes for Therapeutic Angiogenesis in Peripheral Ischemia." in: Tissue engineering. Part A, Vol. 23, Issue 21-22, pp. 1251-1261, (2018) (PubMed).

  • Target See all GPC1 ELISA Kits
    GPC1 (Glypican 1 (GPC1))
    Alternative Name
    Glypican 1 (GPC1 Products)
    Synonyms
    gpc1 ELISA Kit, zgc:63947 ELISA Kit, GPC1 ELISA Kit, Glypican-1 ELISA Kit, glypican ELISA Kit, AI462976 ELISA Kit, HSPG M12 ELISA Kit, HSPGM12 ELISA Kit, glypican 1b ELISA Kit, glypican 1 ELISA Kit, gpc1b ELISA Kit, GPC1 ELISA Kit, gpc1 ELISA Kit, Gpc1 ELISA Kit
    Background
    Gene Names: GPC1
    Protein names: Glypican-1 [Cleaved into: Secreted glypican-1]
    Gene ID
    2817
    UniProt
    P35052
    Pathways
    Glycosaminoglycan Metabolic Process, Regulation of Muscle Cell Differentiation
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