SPINT2 ELISA Kit
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- Target See all SPINT2 ELISA Kits
- SPINT2 (serine Peptidase Inhibitor, Kunitz Type, 2 (SPINT2))
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Reactivity
- Human
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 3-800 pg/mL
- Minimum Detection Limit
- 3 pg/mL
- Application
- ELISA
- Purpose
- Human HAI-2 ELISA Kit for cell culture supernatants, EDTA and/or Citrate treated plasma, and serum samples. Heparin is not recommended as an anticoagulant for use in this assay.
- Sample Type
- Cell Culture Supernatant, Plasma, Serum
- Analytical Method
- Quantitative
- Specificity
- This ELISA antibody pair detects human HAI-2, less than 0.1 % cross-reactivity with human HAI-1 and mouse HAI-2B is observed
- Sensitivity
- 3 pg/mL
- Characteristics
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Components
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Material not included
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- Application Notes
- Recommended Dilution for serum and plasma samples2 fold
- Sample Volume
- 100 μL
- Plate
- Pre-coated
- Protocol
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- Sample Preparation
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Collect plasma using EDTA and/or Citrate as anticoagulants. Heparin is not recommended as an anticoagulant for use in this assay.
- Restrictions
- For Research Use only
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- Storage
- -20 °C
- Storage Comment
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- Expiry Date
- 6 months
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- Target See all SPINT2 ELISA Kits
- SPINT2 (serine Peptidase Inhibitor, Kunitz Type, 2 (SPINT2))
- Alternative Name
- HAI-2 (SPINT2 Products)
- Synonyms
- HAI-2 ELISA Kit, Pb ELISA Kit, DIAR3 ELISA Kit, HAI2 ELISA Kit, Kop ELISA Kit, PB ELISA Kit, AL024025 ELISA Kit, C76321 ELISA Kit, serine peptidase inhibitor, Kunitz type, 2 ELISA Kit, serine peptidase inhibitor, Kunitz type 2 ELISA Kit, serine protease inhibitor, Kunitz type 2 ELISA Kit, Spint2 ELISA Kit, SPINT2 ELISA Kit
- Background
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Gene Names: SPINT2 HAI2 KOP
Protein names: Kunitz-type protease inhibitor 2 (Hepatocyte growth factor activator inhibitor type 2) (HAI-2) (Placental bikunin)
- Gene ID
- 10653
- UniProt
- O43291
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