Midkine ELISA Kit

Catalog No. ABIN2703330

Product Details Midkine ELISA Kit

Target: Midkine (MDK) (Midkine (Neurite Growth-Promoting Factor 2) (MDK))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.12-30 ng/mL

Minimum Detection Limit: 0.12 ng/mL

Application: ELISA

Purpose: Human Midkine ELISA Kit for cell culture supernatants, plasma, and serum samples.

Sample Type: Cell Culture Supernatant, Plasma, Serum

Analytical Method: Quantitative

Specificity: Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: Human ADAM12, ADAM8, B7-H3, BMPR-IB, Cadherin-13, Cadherin-4, CD155, CD229, CD48, CD58, CD84, CD99, CEACAM-5, CF XIV, Cystatin A, Cystatin B, Cystatin E/M, Desmoglein 2, DR3, ErbB4, ESAM, FGF-21, Galectin-2, Galectin-9, ICOS, JAM-A, JAM-B, Kallikrein 5, Midkine, Pentraxin 3, Pref-1, Siglec-10, SLAM, SP-D, Syndecan-4, Testican 2, TIM-3, TLR4, TRAIL, ULBP-1

Sensitivity: 0.12 ng/mL

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Recommended Dilution for serum and plasma samples2 fold

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for Midkine

Target: Midkine (MDK) (Midkine (Neurite Growth-Promoting Factor 2) (MDK))

Alternative Name: Midkine (MDK Products)

Synonyms: ARAP ELISA Kit, MK ELISA Kit, NEGF2 ELISA Kit, Mek ELISA Kit, MK-B ELISA Kit, PTF-alpha-1 ELISA Kit, mdk ELISA Kit, midkine ELISA Kit, negf2 ELISA Kit, RIHB ELISA Kit, cb518 ELISA Kit, fb79b05 ELISA Kit, fj33b02 ELISA Kit, fj48a12 ELISA Kit, mdk2 ELISA Kit, plei2 ELISA Kit, wu:fb79b05 ELISA Kit, wu:fj33b02 ELISA Kit, wu:fj48a12 ELISA Kit, MK-A ELISA Kit, PTF-alpha-2 ELISA Kit, X-PTF-alpha1 ELISA Kit, XMK ELISA Kit, mdk-a ELISA Kit, mdk-b ELISA Kit, midkine ELISA Kit, midkine L homeolog ELISA Kit, midkine (neurite growth-promoting factor 2) ELISA Kit, midkine b ELISA Kit, midkine S homeolog ELISA Kit, MDK ELISA Kit, Mdk ELISA Kit, mdk.L ELISA Kit, mdkb ELISA Kit, mdk.S ELISA Kit

Background: Gene Names: MDK MK1 NEGF2
Protein names: Midkine (MK) (Amphiregulin-associated protein) (ARAP) (Midgestation and kidney protein) (Neurite outgrowth-promoting factor 2) (Neurite outgrowth-promoting protein)

Gene ID: 4192

UniProt: P21741

Pathways: RTK Signaling, M Phase, Skeletal Muscle Fiber Development