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TIM3 ELISA Kit
TIM 3
Reactivity: Human
Colorimetric
Sandwich ELISA
10-2500 pg/mL
Cell Culture Supernatant, Plasma, Serum
Product Details TIM3 ELISA Kit
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Target
See all TIM3 (TIM 3) ELISA Kits
TIM3 (TIM 3)
(Hepatitis A Virus Cellular Receptor 2 (TIM 3))
Reactivity
All reactivities for TIM3 elisa kits
Human
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Detection Range
10-2500 pg/mL
Minimum Detection Limit
10 pg/mL
Application
ELISA
Purpose
Human TIM-3 (KIM-3) ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.
Sample Type
Cell Culture Supernatant, Plasma, Serum
Analytical Method
Quantitative
Specificity
The antibody pair provided in this kit recognizes Human T Cell Immunoglobulin Mucin-3 (HAVCR2 / KIM-3)
Sensitivity
10 pg/mL
Characteristics
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Components
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Material not included
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Application Details
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Application Notes
Recommended Dilution for serum and plasma samples5 fold
Sample Volume
100 μL
Plate
Pre-coated
Protocol
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Handling
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Storage
-20 °C
Storage Comment
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date
6 months
Target Details for TIM3
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Target
See all TIM3 (TIM 3) ELISA Kits
TIM3 (TIM 3)
(Hepatitis A Virus Cellular Receptor 2 (TIM 3))
Alternative Name
TIM-3 (TIM 3 Products )
Synonyms
HAVCR2 ELISA Kit, MGC140131 ELISA Kit, HAVcr-2 ELISA Kit, KIM-3 ELISA Kit, TIM3 ELISA Kit, TIMD-3 ELISA Kit, TIMD3 ELISA Kit, Tim-3 ELISA Kit, TIM-3 ELISA Kit, Tim3 ELISA Kit, Timd3 ELISA Kit, tim3 ELISA Kit, hepatitis A virus cellular receptor 2 ELISA Kit, HAVCR2 ELISA Kit, Havcr2 ELISA Kit
Target Type
Virus
Background
Gene Names: HAVCR2 TIM3 TIMD3 Protein names: Hepatitis A virus cellular receptor 2 (HAVcr-2) (T-cell immunoglobulin and mucin domain-containing protein 3) (TIMD-3) (T-cell immunoglobulin mucin receptor 3) (TIM-3) (T-cell membrane protein 3)
Gene ID
84868
UniProt
Q8TDQ0
Pathways
Regulation of Lipid Metabolism by PPARalpha , Cancer Immune Checkpoints
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