Transferrin ELISA Kit

Catalog No. ABIN2703533

Product Details Transferrin ELISA Kit

Target: Transferrin (TF)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 1-750 ng/mL

Minimum Detection Limit: 1 ng/mL

Application: ELISA

Purpose: Human Transferrin ELISA Kit for cell culture supernatants, plasma, and serum samples.

Sample Type: Cell Culture Supernatant, Plasma, Serum

Analytical Method: Quantitative

Specificity: This ELISA antibody pair detects human Transferrin. Other species not determined.

Sensitivity: 1 ng/mL

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Recommended Dilution for serum and plasma samples20,000 fold

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Sample Preparation:

For example, add 2 μL of serum or plasma into a tube with 198.0 μL 1x Assay Diluent D to prepare a 100-fold diluted sample. Mix thoroughly and then pipette 2 μL of prepared 100-fold diluted sample into a tube with 398 μL 1x Assay Diluent to prepare a final 20,000-fold diluted sample.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for Transferrin

Target: Transferrin (TF)

Alternative Name: Transferrin (TF Products)

Synonyms: ltf ELISA Kit, pro1557 ELISA Kit, pro2086 ELISA Kit, mgc107777 ELISA Kit, LOC692564 ELISA Kit, TF ELISA Kit, LOC100144362 ELISA Kit, tf ELISA Kit, LTF ELISA Kit, TFEW ELISA Kit, conalbumin ELISA Kit, tf-b ELISA Kit, MGC64306 ELISA Kit, PRO1557 ELISA Kit, PRO2086 ELISA Kit, TFQTL1 ELISA Kit, AI266983 ELISA Kit, Cd176 ELISA Kit, HP ELISA Kit, Tf ELISA Kit, Tfn ELISA Kit, hpx ELISA Kit, Trf ELISA Kit, cb285 ELISA Kit, gavi ELISA Kit, id:ibd3238 ELISA Kit, id:ibd3525 ELISA Kit, sb:cb285 ELISA Kit, wu:fb57g06 ELISA Kit, wu:fb62h02 ELISA Kit, wu:fb63h10 ELISA Kit, wu:fb64h10 ELISA Kit, zgc:112154 ELISA Kit, 143958_at ELISA Kit, CG6186 ELISA Kit, Dmel\\CG6186 ELISA Kit, TSF1 ELISA Kit, anon-EST:Posey265 ELISA Kit, tsf1 ELISA Kit, Pro-TRH ELISA Kit, IL-5 ELISA Kit, STF I ELISA Kit, TRF1 ELISA Kit, sTF1 ELISA Kit, sTf ELISA Kit, tf1 ELISA Kit, transferrin ELISA Kit, serotransferrin ELISA Kit, transferrin (ovotransferrin) ELISA Kit, transferrin L homeolog ELISA Kit, melanotransferrin ELISA Kit, transferrin-a ELISA Kit, Transferrin 1 ELISA Kit, thyrotropin releasing hormone ELISA Kit, interleukin 5 ELISA Kit, TF ELISA Kit, LOC477072 ELISA Kit, tf ELISA Kit, Tf ELISA Kit, LOC100144362 ELISA Kit, tf.L ELISA Kit, LOC5575625 ELISA Kit, Trf ELISA Kit, tfa ELISA Kit, Tsf1 ELISA Kit, TRH ELISA Kit, IL5 ELISA Kit, LOC101085148 ELISA Kit, LOC100726872 ELISA Kit, trf ELISA Kit

Background: Gene Names: TF PRO1400
Protein names: Serotransferrin (Transferrin) (Beta-1 metal-binding globulin) (Siderophilin)

Gene ID: 7018

UniProt: P02787

Pathways: Transition Metal Ion Homeostasis