TNFAIP6 ELISA Kit

Catalog No. ABIN2703540

Product Details TNFAIP6 ELISA Kit

Target: TNFAIP6 (Tumor Necrosis Factor-Inducible Protein 6 (TNFAIP6))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.2-50 ng/mL

Minimum Detection Limit: 0.2 ng/mL

Application: ELISA

Purpose: Human TSG-6 ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.

Sample Type: Cell Culture Supernatant, Plasma, Serum

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes human TSG-6. Other species not yet determined.

Sensitivity: 0.2 ng/mL

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Recommended Dilution for serum and plasma samples2 fold

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

References for TNFAIP6 ELISA Kit (ABIN2703540)

Um, Kim, Lee, Song, Seo: "TSG-6 secreted by mesenchymal stem cells suppresses immune reactions influenced by BMP-2 through p38 and MEK mitogen-activated protein kinase pathway." in: Cell and tissue research, Vol. 368, Issue 3, pp. 551-561, (2018) (PubMed).

Kui, Chan, Lee: "TSG-6 Downregulates IFN-Alpha and TNF-Alpha Expression by Suppressing IRF7 Phosphorylation in Human Plasmacytoid Dendritic Cells." in: Mediators of inflammation, Vol. 2017, pp. 7462945, (2017) (PubMed).

Target Details for TNFAIP6

Target: TNFAIP6 (Tumor Necrosis Factor-Inducible Protein 6 (TNFAIP6))

Alternative Name: TSG-6 (TNFAIP6 Products)

Synonyms: Tnfip6 ELISA Kit, TSG-6 ELISA Kit, Tsg6 ELISA Kit, TSG6 ELISA Kit, TNF alpha induced protein 6 ELISA Kit, tumor necrosis factor alpha induced protein 6 ELISA Kit, Tnfaip6 ELISA Kit, TNFAIP6 ELISA Kit

Background: Gene Names: TNFAIP6 TSG6
Protein names: Tumor necrosis factor-inducible gene 6 protein (Hyaluronate-binding protein) (TNF-stimulated gene 6 protein) (TSG-6) (Tumor necrosis factor alpha-induced protein 6) (TNF alpha-induced protein 6)

Gene ID: 7130

UniProt: P98066