Uromodulin ELISA Kit
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- Target See all Uromodulin (UMOD) ELISA Kits
- Uromodulin (UMOD)
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Reactivity
- Human
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 40-10.000 pg/mL
- Minimum Detection Limit
- 40 pg/mL
- Application
- ELISA
- Purpose
- Human Uromodulin ELISA Kit for cell culture supernatants, plasma, and serum samples.
- Sample Type
- Cell Culture Supernatant, Plasma, Serum
- Analytical Method
- Quantitative
- Specificity
- This ELISA antibody pair detects human Uromodulin. Other species not determined.
- Sensitivity
- 40 pg/mL
- Characteristics
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Components
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Material not included
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- Discover our best selling UMOD ELISA Kit
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- Discover our top product UMOD ELISA Kit
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- Application Notes
- Recommended Dilution for serum and plasma samples200 fold
- Sample Volume
- 100 μL
- Plate
- Pre-coated
- Protocol
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- Restrictions
- For Research Use only
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- Storage
- -20 °C
- Storage Comment
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- Expiry Date
- 6 months
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-
Urinary biomarkers of chronic allograft nephropathy." in: Proteomics. Clinical applications, Vol. 9, Issue 5-6, pp. 574-85, (2015) (PubMed).
: "
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Urinary biomarkers of chronic allograft nephropathy." in: Proteomics. Clinical applications, Vol. 9, Issue 5-6, pp. 574-85, (2015) (PubMed).
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- Target See all Uromodulin (UMOD) ELISA Kits
- Uromodulin (UMOD)
- Alternative Name
- Uromodulin (UMOD Products)
- Synonyms
- ADMCKD2 ELISA Kit, FJHN ELISA Kit, HNFJ ELISA Kit, HNFJ1 ELISA Kit, MCKD2 ELISA Kit, THGP ELISA Kit, THP ELISA Kit, urehr4 ELISA Kit, uromodulin ELISA Kit, UMOD ELISA Kit, Umod ELISA Kit
- Background
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Gene Names: UMOD
Protein names: Uromodulin (Tamm-Horsfall urinary glycoprotein) (THP) [Cleaved into: Uromodulin, secreted form]
- Gene ID
- 7369
- UniProt
- P07911
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