Vitronectin ELISA Kit

Catalog No. ABIN2703572

Product Details Vitronectin ELISA Kit

Target: Vitronectin (VTN)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.8-200 ng/mL

Minimum Detection Limit: 0.8 ng/mL

Application: ELISA

Purpose: Human Vitronectin ELISA Kit for cell culture supernatants, plasma, and serum samples.

Sample Type: Cell Culture Supernatant, Plasma, Serum

Analytical Method: Quantitative

Specificity: Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human A2M, ApoA1, ApoC1, ApoC2, ApoC3, ApoE, ApoH, Artemin, BMP-6, Calbindin D, Ck beta 8-1 , CNTF, EMMPRIN, Endostatin, Fibrinogen, FLRG, GITR Ligand, GPBB, Hemoglobin, HTRA2, IL-17E, IL-1R3, Lactoferrin, Mesothelin, PAPP-A, PDECGF, PDGF Ralpha, Pepsinogen II, Periostin, Persephin, PTH, RAP, SAA, Serpin F1, Tie-1, tPA, TSP-2, Ubiquitin+1, Vitronectin, vWF

Sensitivity: 0.8 ng/mL

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Recommended Dilution for serum and plasma samples10,000 fold

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Sample Preparation:

For example, add 2 μL of serum or plasma into a tube with 198.0 μL 1X Assay Diluent to prepare a 100-fold diluted sample. Mix thoroughly and then pipette 2 μL of prepared 100-fold diluted sample into a tube with 198 μL 1X Assay Diluent to prepare a final 10,000-fold diluted sample.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for Vitronectin

Target: Vitronectin (VTN)

Alternative Name: Vitronectin (VTN Products)

Synonyms: VTN ELISA Kit, DKFZp470F0511 ELISA Kit, LOC100228142 ELISA Kit, vtn ELISA Kit, zgc:112277 ELISA Kit, V75 ELISA Kit, VN ELISA Kit, VNT ELISA Kit, AI256434 ELISA Kit, Vn ELISA Kit, Aa1018 ELISA Kit, vitronectin ELISA Kit, vitronectin a ELISA Kit, vitronectin b ELISA Kit, vitronectin L homeolog ELISA Kit, VTN ELISA Kit, vtn ELISA Kit, vtna ELISA Kit, vtnb ELISA Kit, CpipJ_CPIJ016691 ELISA Kit, vtn.L ELISA Kit, Vtn ELISA Kit

Background: Gene Names: VTN
Protein names: Vitronectin (VN) (S-protein) (Serum-spreading factor) (V75) [Cleaved into: Vitronectin V65 subunit, Vitronectin V10 subunit, Somatomedin-B]

Gene ID: 7448

UniProt: P04004

Pathways: Autophagy, Smooth Muscle Cell Migration