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SMAD1 ELISA Kit

SMAD1 Reactivity: Human pSer465 Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
Catalog No. ABIN2747895
  • Target See all SMAD1 ELISA Kits
    SMAD1 (SMAD, Mothers Against DPP Homolog 1 (SMAD1))
    Binding Specificity
    pSer463, pSer465, total
    Reactivity
    • 9
    • 7
    • 6
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Application
    ELISA
    Purpose
    Human Phospho-SMAD1 (SER463/465) and Total SMAD1 Kit. This assay semi-quantitatively measures phophorylated SMAD1 (Ser463/465) and Total SMAD1 in lysate samples.
    Sample Type
    Cell Lysate, Tissue Lysate
    Analytical Method
    Semi-Quantitative
    Specificity
    The antibody pair provided in this kit recognizes human SMAD1 phosphorylated at site Serine-463/465 and Total SMAD1.
    Characteristics
    • Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
    • Screen numerous different cell lysates without performing a Western Blot analysis
    • Minimal hands-on time, convenient, and non-radioactive material
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Anti-Phospho Antibody
    • Anti-Pan Antibody
    • HRP-Conjugated Secondary Antibody
    • Streptavidin-Conjugated HRP
    • Assay Diluent
    • TMB One-Step Substrate
    • Stop Solution
    • Lysis Buffer
    • Positive Control Sample
    Material not included
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
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  • Sample Volume
    100 μL
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Assay Procedure

    Prepare all reagents and samples as instructed in the manual.
    Add 100 μL of sample or positive control to each well.
    Incubate 2.5 h at RT or O/N at 4 °C.
    Add 100 μL of prepared primary antibody to each well.
    Incubate 1 h at RT.
    Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    Incubate 1 h at RT.
    Add 100 μL of TMB One-Step Substrate Reagent to each well.
    Incubate 30 min at RT.
    Add 50 μL of Stop Solution to each well.
    Read at 450 nm immediately.

    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
    Expiry Date
    6 months
  • Target See all SMAD1 ELISA Kits
    SMAD1 (SMAD, Mothers Against DPP Homolog 1 (SMAD1))
    Alternative Name
    SMAD1 (SMAD1 Products)
    Synonyms
    MLP ELISA Kit, Xmad1 ELISA Kit, Xsmad1 ELISA Kit, bsp1 ELISA Kit, jv4-1 ELISA Kit, jv41 ELISA Kit, madh1 ELISA Kit, madr1 ELISA Kit, smad-1 ELISA Kit, MADH1 ELISA Kit, fb39h09 ELISA Kit, wu:fb39h09 ELISA Kit, wu:fb97e04 ELISA Kit, 2/23 ELISA Kit, CG12399 ELISA Kit, Dmel\\CG12399 ELISA Kit, E(zen)2 ELISA Kit, En(vvl) ELISA Kit, MAD ELISA Kit, Mat ELISA Kit, P-Mad ELISA Kit, P-mad ELISA Kit, PMad ELISA Kit, Smad ELISA Kit, Smad1 ELISA Kit, apg ELISA Kit, c28 ELISA Kit, dMAD ELISA Kit, dMad ELISA Kit, l(2)K00237 ELISA Kit, l(2)k00237 ELISA Kit, mad ELISA Kit, p-Mad ELISA Kit, pMAD ELISA Kit, pMad ELISA Kit, pSmad ELISA Kit, BSP-1 ELISA Kit, BSP1 ELISA Kit, JV4-1 ELISA Kit, JV41 ELISA Kit, MADR1 ELISA Kit, AI528653 ELISA Kit, Madh1 ELISA Kit, Madr1 ELISA Kit, Mlp1 ELISA Kit, MusMLP ELISA Kit, smad1 ELISA Kit, xmad ELISA Kit, SMAD family member 1 ELISA Kit, Mothers against dpp ELISA Kit, SMAD family member 1 L homeolog ELISA Kit, smad1 ELISA Kit, SMAD1 ELISA Kit, Mad ELISA Kit, Smad1 ELISA Kit, smad1.L ELISA Kit
    Background
    Mothers against decapentaplegic homolog 1 (SMAD1) phosphorylated at Serine-463/465 and Total SMAD1
    Gene ID
    4086
    UniProt
    Q15797
    Pathways
    Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development
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