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Src ELISA Kit

Src Reactivity: Human pTyr419 Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
Catalog No. ABIN2747907
  • Target See all Src ELISA Kits
    Src (Proto-oncogene tyrosine-protein kinase Src (Src))
    Binding Specificity
    pTyr419
    Reactivity
    • 9
    • 5
    • 5
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Application
    ELISA
    Purpose
    Human Phospho-SRC (Y419) ELISA Kit. This assay semi-quantitatively measures phophorylated SRC (Tyr419) in lysate samples.
    Sample Type
    Cell Lysate, Tissue Lysate
    Analytical Method
    Semi-Quantitative
    Specificity
    The antibody pair provided in this kit recognizes human SRC phosphorylated at site tyrosine 419.
    Characteristics
    • Rapidly measure phosphorylated protein in lysates
    • Screen numerous different cell lysates without performing a Western Blot analysis
    • Minimal hands-on time, convenient, and non-radioactive material
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Anti-Phospho Antibody
    • HRP-Conjugated Secondary Antibody
    • Assay Diluent
    • TMB One-Step Substrate
    • Stop Solution
    • Lysis Buffer
    • Positive Control Sample
    Material not included
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
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  • Sample Volume
    100 μL
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Assay Procedure

    Prepare all reagents and samples as instructed in the manual.
    Add 100 μL of sample or positive control to each well.
    Incubate 2.5 h at RT or O/N at 4 °C.
    Add 100 μL of prepared primary antibody to each well.
    Incubate 1 h at RT.
    Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    Incubate 1 h at RT.
    Add 100 μL of TMB One-Step Substrate Reagent to each well.
    Incubate 30 min at RT.
    Add 50 μL of Stop Solution to each well.
    Read at 450 nm immediately.

    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
    Expiry Date
    6 months
  • Target See all Src ELISA Kits
    Src (Proto-oncogene tyrosine-protein kinase Src (Src))
    Alternative Name
    SRC (Src Products)
    Synonyms
    AW259666 ELISA Kit, pp60c-src ELISA Kit, C-src1 ELISA Kit, CG7524 ELISA Kit, D-Src64B ELISA Kit, D-src ELISA Kit, DSRC64 ELISA Kit, DSrc ELISA Kit, DSrc64 ELISA Kit, DSrc64B ELISA Kit, Dm SRC1 ELISA Kit, DmHD-358 ELISA Kit, Dmel\CG7524 ELISA Kit, Dsrc ELISA Kit, Dsrc64 ELISA Kit, Dsrc64B ELISA Kit, HD-358 ELISA Kit, MRE5 ELISA Kit, SRC 64B ELISA Kit, Src ELISA Kit, Src1 ELISA Kit, Src64 ELISA Kit, c-src ELISA Kit, dSrc ELISA Kit, dsrc ELISA Kit, src ELISA Kit, src1 ELISA Kit, src64 ELISA Kit, src64B ELISA Kit, src64b ELISA Kit, ASV ELISA Kit, SRC1 ELISA Kit, c-SRC ELISA Kit, p60-Src ELISA Kit, c-Src ELISA Kit, PP60C-SCR ELISA Kit, SDR ELISA Kit, SRC proto-oncogene, non-receptor tyrosine kinase ELISA Kit, uncharacterized LOC5574802 ELISA Kit, proto-oncogene tyrosine-protein kinase src ELISA Kit, Rous sarcoma oncogene ELISA Kit, Src oncogene at 64B ELISA Kit, SRC ELISA Kit, LOC5574802 ELISA Kit, CpipJ_CPIJ010659 ELISA Kit, Src ELISA Kit, Src64B ELISA Kit
    Target Type
    Viral Protein
    Background
    Phospho-SRC (Y419). Full Name = Proto-oncogene tyrosine-protein kinase Src (P12931)
    Gene ID
    6714
    UniProt
    P12931
    Pathways
    JAK-STAT Signaling, Neurotrophin Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Cellular Response to Molecule of Bacterial Origin, Cell-Cell Junction Organization, Regulation of Carbohydrate Metabolic Process, Autophagy, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling, Platelet-derived growth Factor Receptor Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling
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