SRC ELISA Kit (V-Src Sarcoma (Schmidt-Ruppin A-2) Viral Oncogene Homolog (Avian))

Details for Product SRC ELISA Kit No. ABIN2747908, Supplier: Log in to see
Antigen
  • AW259666
  • pp60c-src
  • C-src1
  • CG7524
  • D-Src64B
  • D-src
  • DSRC64
  • DSrc
  • DSrc64
  • DSrc64B
  • Dm SRC1
  • DmHD-358
  • Dmel\CG7524
  • Dsrc
  • Dsrc64
  • Dsrc64B
  • HD-358
  • MRE5
  • SRC 64B
  • Src
  • Src1
  • Src64
  • c-src
  • dSrc
  • dsrc
  • src
  • src1
  • src64
  • src64B
  • src64b
  • ASV
  • SRC1
  • c-SRC
  • p60-Src
  • c-Src
  • PP60C-SCR
  • SDR
  • SRC proto-oncogene, non-receptor tyrosine kinase
  • uncharacterized LOC5574802
  • proto-oncogene tyrosine-protein kinase src
  • Rous sarcoma oncogene
  • Src oncogene at 64B
  • SRC
  • LOC5574802
  • CpipJ_CPIJ010659
  • Src
  • Src64B
Epitope
pTyr419, total
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
14
7
7
Method Type
Sandwich ELISA
Application
ELISA
Options
Supplier
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Supplier Product No.
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Purpose Human Phospho-SRC (Y419) and Total SRC ELISA Kit. This assay semi-quantitatively measures phophorylated SRC (Tyr419) and Total SRC in lysate samples.
Sample Type Cell Lysate, Tissue Lysate
Analytical Method Semi-Quantitative
Detection Method Colorimetric
Specificity The antibody pair provided in this kit recognizes Human SRC phosphorylated at site Tyrosin-419 and Total SRC
Characteristics
  • Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
  • Screen numerous different cell lysates without performing a Western Blot analysis
  • Minimal hands-on time, convenient, and non-radioactive material
Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Anti-Phospho Antibody
  • Anti-Pan Antibody
  • HRP-Conjugated Secondary Antibody
  • Streptavidin-Conjugated HRP
  • Assay Diluent
  • TMB One-Step Substrate
  • Stop Solution
  • Lysis Buffer
  • Positive Control Sample
Material not included
  • Distilled or deionized water
  • 100 mL and 1 liter graduated cylinders
  • Tubes to prepare sample dilutions
  • Protease and Phosphatase inhibitors
  • Precision pipettes to deliver 2 μL to 1 mL volumes
  • Adjustable 1-25 mL pipettes for reagent preparation
  • Benchtop rocker or shaker
  • Microplate reader capable of measuring absorbance at 450 nm
Alternative Name SRC (SRC ELISA Kit Abstract)
Background Phospho-SRC (Y419). Full Name = Proto-oncogene tyrosine-protein kinase Src (P12931)
Gene ID 6714
UniProt P12931
Pathways JAK-STAT Signaling, Neurotrophin Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Cellular Response to Molecule of Bacterial Origin, Cell-Cell Junction Organization, Regulation of Carbohydrate Metabolic Process, Autophagy, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling, Platelet-derived growth Factor Receptor Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling
Sample Volume 100 μL
Plate Pre-coated
Protocol
  1. Prepare all reagents and samples as instructed in the manual.
  2. Add 100 μL of sample or positive control to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 μL of prepared primary antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
  7. Incubate 1 h at RT.
  8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 μL of Stop Solution to each well.
  11. Read at 450 nm immediately.
Assay Procedure

Prepare all reagents and samples as instructed in the manual.
Add 100 μL of sample or positive control to each well.
Incubate 2.5 h at RT or O/N at 4 °C.
Add 100 μL of prepared primary antibody to each well.
Incubate 1 h at RT.
Add 100 μL of prepared 1X HRP-Streptavidin to each well.
Incubate 1 h at RT.
Add 100 μL of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 μL of Stop Solution to each well.
Read at 450 nm immediately.

Restrictions For Research Use only
Storage -20 °C
Storage Comment Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
Expiry Date 6 months
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