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GFRA1 ELISA Kit

GFRA1 Reactivity: Rat Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Catalog No. ABIN2748144
  • Target See all GFRA1 ELISA Kits
    GFRA1 (GDNF Family Receptor alpha 1 (GFRA1))
    Reactivity
    • 4
    • 3
    • 1
    • 1
    • 1
    Rat
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Application
    ELISA
    Purpose
    Custom Rat GFR alpha-1 (GDNFR) ELISA Kit.
    Sample Type
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Specificity
    The antibody pair provided in this kit recognizes Rat GFR alpha-1 (GDNFR)
    Characteristics
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Material not included
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Sample Volume
    100 μL
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Assay Procedure

    Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.

    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Expiry Date
    6 months
  • Target See all GFRA1 ELISA Kits
    GFRA1 (GDNF Family Receptor alpha 1 (GFRA1))
    Alternative Name
    GFR alpha-1 (GFRA1 Products)
    Synonyms
    gdnfr ELISA Kit, ret1l ELISA Kit, retl1 ELISA Kit, trnr1 ELISA Kit, gdnfra ELISA Kit, MGC122616 ELISA Kit, gfr-alpha-1 ELISA Kit, GDNFR ELISA Kit, GDNFRA ELISA Kit, GFR-ALPHA-1 ELISA Kit, RET1L ELISA Kit, RETL1 ELISA Kit, TRNR1 ELISA Kit, GDNFRALPHA ELISA Kit, GFRA1 ELISA Kit, AU042498 ELISA Kit, gfralpha1a ELISA Kit, zgc:110129 ELISA Kit, GDNF family receptor alpha 1 ELISA Kit, glial cell line derived neurotrophic factor family receptor alpha 1 ELISA Kit, gdnf family receptor alpha 1a ELISA Kit, gfra1 ELISA Kit, GFRA1 ELISA Kit, Gfra1 ELISA Kit, gfra1a ELISA Kit
    Background
    GFR alpha-1 (GDNFR)
    Gene ID
    25454
    UniProt
    Q62997
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