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H2AFX ELISA Kit

H2AFX Reactivity: Human pSer139 Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
Catalog No. ABIN2748158
  • Target See all H2AFX ELISA Kits
    H2AFX (H2A Histone Family, Member X (H2AFX))
    Binding Specificity
    pSer139
    Reactivity
    • 5
    • 3
    • 2
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Application
    ELISA
    Purpose
    Human Phospho-H2AX (S139) ELISA Kit. This assay semi-quantitatively measures phophorylated H2AX (Ser139) in lysate samples.
    Sample Type
    Cell Lysate, Tissue Lysate
    Analytical Method
    Semi-Quantitative
    Specificity
    The antibody pair provided in this kit recognizes human H2AX phosphorylated at Serine-139 and total H2AX.
    Characteristics
    • Rapidly measure phosphorylated protein in lysates
    • Screen numerous different cell lysates without performing a Western Blot analysis
    • Minimal hands-on time, convenient, and non-radioactive material
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Anti-Phospho Antibody
    • HRP-Conjugated Secondary Antibody
    • Assay Diluent
    • TMB One-Step Substrate
    • Stop Solution
    • Lysis Buffer
    • Positive Control Sample
    Material not included
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
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  • Sample Volume
    100 μL
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Assay Procedure

    Prepare all reagents and samples as instructed in the manual.
    Add 100 μL of sample or positive control to each well.
    Incubate 2.5 h at RT or O/N at 4 °C.
    Add 100 μL of prepared primary antibody to each well.
    Incubate 1 h at RT.
    Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    Incubate 1 h at RT.
    Add 100 μL of TMB One-Step Substrate Reagent to each well.
    Incubate 30 min at RT.
    Add 50 μL of Stop Solution to each well.
    Read at 450 nm immediately.

    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
    Expiry Date
    6 months
  • Target See all H2AFX ELISA Kits
    H2AFX (H2A Histone Family, Member X (H2AFX))
    Alternative Name
    H2AX (H2AFX Products)
    Synonyms
    H2A.X ELISA Kit, H2A/X ELISA Kit, H2AX ELISA Kit, AW228881 ELISA Kit, H2ax ELISA Kit, Hist5-2ax ELISA Kit, gammaH2ax ELISA Kit, zgc:56329 ELISA Kit, h2a.x ELISA Kit, h2a/x ELISA Kit, h2ax ELISA Kit, RGD1566119 ELISA Kit, h2a ELISA Kit, h2afx ELISA Kit, H2A histone family member X ELISA Kit, H2A histone family, member X ELISA Kit, H2A histone family member X L homeolog ELISA Kit, histone cluster 1, H2ah ELISA Kit, histone cluster 2, H2ab S homeolog ELISA Kit, histone H2AX ELISA Kit, H2AFX ELISA Kit, H2afx ELISA Kit, h2afx ELISA Kit, h2afx.L ELISA Kit, HIST1H2AH ELISA Kit, hist2h2ab.S ELISA Kit, LOC100522201 ELISA Kit, LOC100720536 ELISA Kit
    Background
    Histone H2AX phosphorylated at Serine-S139
    Gene ID
    3014
    UniProt
    P16104
    Pathways
    Telomere Maintenance, DNA Damage Repair, Positive Regulation of Response to DNA Damage Stimulus
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