HGF ELISA Kit (Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor))

Details for Product HGF ELISA Kit No. ABIN2748164, Supplier: Log in to see
Antigen
  • DFNB39
  • F-TCF
  • HGFB
  • HPTA
  • SF
  • HGF
  • C230052L06Rik
  • HGF/SF
  • NK1
  • NK2
  • SF/HGF
  • hepatocyte growth factor
  • HGF
  • Hgf
Reactivity
Rat (Rattus)
Alternatives
Kits with alternative reactivity to:
37
23
21
15
12
11
6
2
2
1
1
1
1
1
Method Type
Sandwich ELISA
Detection Range
0.8-200 ng/mL
Minimum Detection Limit
0.8 ng/mL
Application
ELISA
Options
Supplier
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Supplier Product No.
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Purpose Rat HGF ELISA Kit for cell culture supernatants, plasma, and serum samples.
Sample Type Plasma, Cell Culture Supernatant, Serum
Analytical Method Quantitative
Detection Method Colorimetric
Specificity Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: rat 4-1BB, Activin A, Adiponectin, B7-1, CD48, CTACK, Decorin, Eotaxin, EphA5, Erythropoietin, FGF-BP, Fit-3 Ligand, Galectin-1, Galectin-3, Gas 1, GFR alpha-1, gp130, IL-1 R6, IL-1 ra, IL-2 R alpha, IL-3, IL-7, IL-17F, IL-22, JAM-A, MIP-1 alpha, Neuropilin-1, Neuropilin-2, Nope, Notch-1, Notch-2, P-Cadherin, Prolactin, RANTES, SCF, TIM-1, TIMP-2, TREM-1, TWEAK R.
Sensitivity 0.8 ng/mL
Characteristics
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data
Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Material not included
  • Distilled or deionized water
  • Precision pipettes to deliver 2 μL to 1 μL volumes
  • Adjustable 1-25 μL pipettes for reagent preparation
  • 100 μL and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Alternative Name HGF (HGF ELISA Kit Abstract)
Background Hepatocyte growth factor (Hepatopoietin-A) (Scatter factor) (SF)
Gene ID 24446
UniProt P17945
Pathways RTK Signaling, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Synaptic Membrane, Signaling of Hepatocyte Growth Factor Receptor
Application Notes Recommended Dilution for serum and plasma samples2 fold
Sample Volume 100 μL
Plate Pre-coated
Protocol
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 μL of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 μL of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 μL of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 μL of Stop Solution to each well.
  11. Read at 450 nm immediately.
Assay Procedure

Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.

Restrictions For Research Use only
Storage -20 °C
Storage Comment The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date 6 months
Supplier Images
ELISA image for Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF) ELISA Kit (ABIN2748164) Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF) ELISA Kit
Product cited in: Deegan, Zimmerman, Skardal, Atala, Shupe: "Stiffness of hyaluronic acid gels containing liver extracellular matrix supports human hepatocyte function and alters cell morphology." in: Journal of the mechanical behavior of biomedical materials, Vol. 55, pp. 87-103, 2015 (PubMed).

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