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RPS6KA1 ELISA Kit

RPS6KA1 Reactivity: Human pSer380 Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
Catalog No. ABIN2748547
  • Target See all RPS6KA1 ELISA Kits
    RPS6KA1 (Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1 (RPS6KA1))
    Binding Specificity
    pSer380
    Reactivity
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Application
    ELISA
    Purpose
    Human Phosph-RSK1 (S380) ELISA Kit. This assay semi-quantitatively measures phophorylated RSK1 (Ser380) in lysate samples.
    Sample Type
    Cell Lysate, Tissue Lysate
    Analytical Method
    Semi-Quantitative
    Specificity
    The antibody pair provided in this kit recognizes human RSK1 phosphorylated at site Serine-380 .
    Characteristics
    • Rapidly measure phosphorylated protein in lysates
    • Screen numerous different cell lysates without performing a Western Blot analysis
    • Minimal hands-on time, convenient, and non-radioactive material
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Anti-Phospho Antibody
    • HRP-Conjugated Secondary Antibody
    • Assay Diluent
    • TMB One-Step Substrate
    • Stop Solution
    • Lysis Buffer
    • Positive Control Sample
    Material not included
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
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  • Sample Volume
    100 μL
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Assay Procedure

    Prepare all reagents and samples as instructed in the manual.
    Add 100 μL of sample or positive control to each well.
    Incubate 2.5 h at RT or O/N at 4 °C.
    Add 100 μL of prepared primary antibody to each well.
    Incubate 1 h at RT.
    Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    Incubate 1 h at RT.
    Add 100 μL of TMB One-Step Substrate Reagent to each well.
    Incubate 30 min at RT.
    Add 50 μL of Stop Solution to each well.
    Read at 450 nm immediately.

    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
    Expiry Date
    6 months
  • Target See all RPS6KA1 ELISA Kits
    RPS6KA1 (Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1 (RPS6KA1))
    Alternative Name
    RSK1 (RPS6KA1 Products)
    Synonyms
    HU-1 ELISA Kit, MAPKAPK1A ELISA Kit, RSK ELISA Kit, RSK1 ELISA Kit, Rsk1 ELISA Kit, p90rsk ELISA Kit, rsk ELISA Kit, RPS6KA ELISA Kit, hu-1 ELISA Kit, mapkapk1a ELISA Kit, rsk1 ELISA Kit, wu:fc56h07 ELISA Kit, zgc:152654 ELISA Kit, ribosomal protein S6 kinase A1 ELISA Kit, ribosomal protein S6 kinase polypeptide 1 ELISA Kit, ribosomal protein S6 kinase A1-like ELISA Kit, ribosomal protein S6 kinase A1 L homeolog ELISA Kit, ribosomal protein S6 kinase a, polypeptide 1 ELISA Kit, RPS6KA1 ELISA Kit, Rps6ka1 ELISA Kit, RPS6KA1L ELISA Kit, rps6ka1.L ELISA Kit, rps6ka1 ELISA Kit
    Background
    Phospho-RSK1 (S380)
    Gene ID
    6195
    UniProt
    Q15418
    Pathways
    MAPK Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades
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