RPS6 ELISA Kit (Ribosomal Protein S6)

Details for Product RPS6 ELISA Kit No. ABIN2748618, Supplier: Log in to see
Antigen
  • S6
  • rps6
  • rps6a
  • S6R
  • (Rp)S6
  • CG10944
  • DS6
  • Dmel\\CG10944
  • M(1)7BC
  • M(1)7C
  • RPS6
  • Rp S6
  • Rps6
  • air8
  • air[8]
  • anon-WO02059370.61
  • hen
  • l(1)air8
  • l(1)air[8]
  • l(1)hen
  • pp30
  • rpS6
  • wu:fa92e06
  • wu:fb64g06
  • zgc:92237
  • ribosomal protein S6
  • ribosomal protein S6 S homeolog
  • S6 ribosomal protein
  • Ribosomal protein S6
  • 30S ribosomal protein S6
  • 40S ribosomal protein S6
  • RPS6
  • rps6.S
  • LOC100135859
  • Rps6
  • RpS6
  • rps6
  • rps-6
  • LOC100533219
Alternatives
Human RPS6 ELISA Kit
Epitope
pSer235, pSer236
Reactivity
Human, Mouse (Murine), Rat (Rattus)
Alternatives
Kits with alternative reactivity to:
12
10
9
1
1
1
1
1
1
1
1
1
Method Type
Sandwich ELISA
Application
ELISA
Options
Supplier
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Supplier Product No.
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Purpose Human/Mouse/Rat Phospho-RPS6 (S235/S236) ELISA Kit. This assay semi-quantitatively measures phophorylated RPS6 (Ser235/S236) in lysate samples.
Sample Type Cell Lysate, Tissue Lysate
Analytical Method Semi-Quantitative
Detection Method Colorimetric
Specificity The antibody pair provided in this kit recognizes human, mouse, or rat Phospho-RPS6 (pSer235/Ser236).
Characteristics
  • Rapidly measure phosphorylated protein in lysates
  • Screen numerous different cell lysates without performing a Western Blot analysis
  • Minimal hands-on time, convenient, and non-radioactive material
Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Anti-Phospho Antibody
  • HRP-Conjugated Secondary Antibody
  • Assay Diluent
  • TMB One-Step Substrate
  • Stop Solution
  • Lysis Buffer
  • Positive Control Sample
Material not included
  • Distilled or deionized water
  • 100 mL and 1 liter graduated cylinders
  • Tubes to prepare sample dilutions
  • Protease and Phosphatase inhibitors
  • Precision pipettes to deliver 2 μL to 1 mL volumes
  • Adjustable 1-25 mL pipettes for reagent preparation
  • Benchtop rocker or shaker
  • Microplate reader capable of measuring absorbance at 450 nm
Alternative Name RPS6 (RPS6 ELISA Kit Abstract)
Background Ribosomal protein S6 phosphorylated at S235/S236
Gene ID 6194
UniProt P62753
Research Area Chromatin and Nuclear Signaling, DNA/RNA, Apoptosis/Necrosis, Translation Factors
Pathways Carbohydrate Homeostasis, Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly
Sample Volume 100 μL
Plate Pre-coated
Protocol
  1. Prepare all reagents and samples as instructed in the manual.
  2. Add 100 μL of sample or positive control to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 μL of prepared primary antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
  7. Incubate 1 h at RT.
  8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 μL of Stop Solution to each well.
  11. Read at 450 nm immediately.
Assay Procedure

Prepare all reagents and samples as instructed in the manual.
Add 100 μL of sample or positive control to each well.
Incubate 2.5 h at RT or O/N at 4 °C.
Add 100 μL of prepared primary antibody to each well.
Incubate 1 h at RT.
Add 100 μL of prepared 1X HRP-Streptavidin to each well.
Incubate 1 h at RT.
Add 100 μL of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 μL of Stop Solution to each well.
Read at 450 nm immediately.

Restrictions For Research Use only
Storage -20 °C
Storage Comment Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
Expiry Date 6 months
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