SKP1 ELISA Kit

Catalog No. ABIN2851529

Product Details SKP1 ELISA Kit

Target: SKP1 (S-Phase Kinase-Associated Protein 1 (SKP1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Purpose: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of SKP1 in human tissue homogenates, cell lysates and other biological fluids.

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of this index.

Cross-Reactivity (Details): No significant cross-reactivity or interference between this index and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between this index and all the analogues, therefore, cross reaction may still exist.

Sensitivity: 0.054 ng/mL

Components:

• Pre-coated, ready to use 96-well strip plate
• Standard (freeze dried)
• Standard Diluent
• Detection Reagent A
• Detection Reagent B
• Assay Diluent A
• Assay Diluent B
• TMB
• Stop Solution
• Wash Buffer (30X)
• Plate sealer for 96 wells
• Instruction manual

Material not included:

1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Application Details

Sample Volume: 100 μL

Assay Time: 1 - 4.5 h

Plate: Pre-coated

Protocol: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8. Add 50µL Stop Solution. Read at 450nm immediately.

Assay Procedure:

The microtiter plate provided in this kit has been pre-coated with an antibody specific to the index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to the index. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of the index in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision:

• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
• CV(%) = SD/meanX100
• Intra-assay: CV<10%
• Inter-assay: CV<12%

Restrictions: For Research Use only

Handling

Precaution of Use: The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Handling Advice: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.

Storage: 4 °C,-20 °C

Storage Comment: The Assay Plate, Standard, Detection Reagent A and Detection Reagent B should be stored at -20°C upon being received. After receiving the kit , Substrate should be always stored at 4°C.Other reagents are kept according to the labels on vials. But for long term storage, please keep the whole kit at -20°C. The unused strips should be kept in a sealed bag with the desiccant provided to minimize exposure to damp air. The test kit may be used throughout the expiration date of the kit (six months from the date of manufacture). Opened test kits will remain stable until the expiring date shown, provided it is stored as prescribed above.

Expiry Date: 12 months

Target Details for SKP1

Target: SKP1 (S-Phase Kinase-Associated Protein 1 (SKP1))

Alternative Name: SKP1 (SKP1 Products)

Synonyms: EMC19 ELISA Kit, OCP-II ELISA Kit, OCP2 ELISA Kit, SKP1A ELISA Kit, TCEB1L ELISA Kit, p19A ELISA Kit, 15kDa ELISA Kit, 2610043E24Rik ELISA Kit, 2610206H23Rik ELISA Kit, SKP1 ELISA Kit, Tceb1l ELISA Kit, p19Skp1 ELISA Kit, Skp1a ELISA Kit, skp1a ELISA Kit, zgc:73186 ELISA Kit, ocp2 ELISA Kit, emc19 ELISA Kit, ocp-ii ELISA Kit, tceb1l ELISA Kit, ARABIDOPSIS SKP1 HOMOLOGUE 1 ELISA Kit, ASK1 ELISA Kit, ATSKP1 ELISA Kit, S phase kinase-associated protein 1 ELISA Kit, T4O12.17 ELISA Kit, T4O12_17 ELISA Kit, UFO INTERACTING PROTEIN 1 ELISA Kit, UIP1 ELISA Kit, ocp-2 ELISA Kit, S-phase kinase associated protein 1 ELISA Kit, S-phase kinase-associated protein 1 ELISA Kit, S-phase kinase-associated protein 1 pseudogene ELISA Kit, S-phase kinase-associated protein 1A ELISA Kit, S-phase kinase-associated protein 1 S homeolog ELISA Kit, S phase kinase-associated protein 1 ELISA Kit, SKP1 ELISA Kit, skp1 ELISA Kit, LOC100340043 ELISA Kit, Skp1a ELISA Kit, Skp1 ELISA Kit, skp1.S ELISA Kit

Background: Alternative name: SKP1A, EMC19, OCP-II, OCP2, TCEB1L, p19A, Cyclin-A/CDK2-associated p19, RNA polymerase II elongation factor-like protein, Transcription elongation factor B polypeptide 1-like

Gene ID: 6500

UniProt: P63208

Pathways: Cell Division Cycle, Hedgehog Signaling, Mitotic G1-G1/S Phases