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ERBB3 ELISA Kit

ERBB3 Reactivity: Human AA 20-643 Colorimetric Sandwich ELISA 156-10.000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Catalog No. ABIN2859250
  • Target See all ERBB3 ELISA Kits
    ERBB3 (Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3))
    Binding Specificity
    AA 20-643
    Reactivity
    • 9
    • 3
    • 2
    • 2
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    156-10.000 pg/mL
    Minimum Detection Limit
    156 pg/mL
    Application
    ELISA
    Purpose
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human ERBB3/Her3
    Brand
    PicoKine™
    Sample Type
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytical Method
    Quantitative
    Specificity
    NSO, S20-T643
    Cross-Reactivity (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivity
    <10pg/mL
    Material not included
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: NSO
    Immunogen sequence: S20-T643
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  • Application Notes
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Comment

    Tissue Specificity: Epithelial tissues and brain.

    Plate
    Pre-coated
    Protocol
    human ERBB3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for ERBB3 has been precoated onto 96-well plates. Standards (NSO, S20-T643) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ERBB3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human ERBB3 amount of sample captured in plate.
    Assay Procedure

    Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human ERBB3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human ERBB3 standard solution and each sample be measured in duplicate.

    Assay Precision
    • Sample 1: n=16, Mean(ng/ml): 1.65, Standard deviation: 0.086, CV(%): 5.2
    • Sample 2: n=16, Mean(ng/ml): 4.4, Standard deviation: 0.198, CV(%): 4.5
    • Sample 3: n=16, Mean(ng/ml): 6.25, Standard deviation: 0.294, CV(%): 4.7,
    • Sample 1: n=24, Mean(ng/ml): 1.37, Standard deviation: 0.081, CV(%): 5.9
    • Sample 2: n=24, Mean(ng/ml): 3.56, Standard deviation: 0.182, CV(%): 5.1
    • Sample 3: n=24, Mean(ng/ml): 7.19, Standard deviation: 0.446, CV(%): 6.2
    Restrictions
    For Research Use only
  • Handling Advice
    Avoid multiple freeze-thaw cycles.
    Storage
    -20 °C,4 °C
    Storage Comment
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Expiry Date
    12 months
  • Target See all ERBB3 ELISA Kits
    ERBB3 (Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3))
    Alternative Name
    ERBB3 (ERBB3 Products)
    Synonyms
    ErbB-3 ELISA Kit, HER3 ELISA Kit, LCCS2 ELISA Kit, MDA-BF-1 ELISA Kit, c-erbB-3 ELISA Kit, c-erbB3 ELISA Kit, erbB3-S ELISA Kit, p180-ErbB3 ELISA Kit, p45-sErbB3 ELISA Kit, p85-sErbB3 ELISA Kit, ERBB3 ELISA Kit, C76256 ELISA Kit, Erbb-3 ELISA Kit, Erbb3r ELISA Kit, Her3 ELISA Kit, nuc-ErbB3 ELISA Kit, erb-b2 receptor tyrosine kinase 3 ELISA Kit, v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian) ELISA Kit, ERBB3 ELISA Kit, Erbb3 ELISA Kit
    Background

    Protein Function: Binds and is activated by neuregulins and NTAK. May also be activated by CSPG5. .

    Background: Receptor tyrosine-protein kinase erbB-3, also known as HER3 (human epidermal growth factor receptor 3), is a membrane bound protein that in humans is encoded by the ERBB3 gene. ErbB3 has been shown to bind the ligands heregulin and NRG-2. Ligand binding causes a change in conformation that allows for dimerization, phosphorylation, and activation of signal transduction. ErbB3 can heterodimerize with any of the other three ErbB family members. The theoretical ErbB3 homodimer would be non-functional because the kinase-impaired protein requires transphosporylation by its binding partner to be active. Unlike the other ErbB receptor tyrosine kinase family members which are activated through autophosphorylation upon ligand binding, ErbB3 is found to be kinase impaired, having only 1/1000th the autophosphorylation activity of EGFR and no ability to phosphorylate other proteins. Therefore, ErbB3 must act as anallosteric activator.

    Synonyms: Receptor tyrosine-protein kinase erbB-3,2.7.10.1,Proto-oncogene-like protein c-ErbB-3,Tyrosine kinase-type cell surface receptor HER3,ERBB3,HER3,

    Full Gene Name: Receptor tyrosine-protein kinase erbB-3

    Cellular Localisation: Isoform 1: Cell membrane, Single-pass type I membrane protein.
    Gene ID
    2065
    UniProt
    P21860
    Pathways
    RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
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