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TDGF1 ELISA Kit

TDGF1 Reactivity: Mouse AA 26-150 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Catalog No. ABIN2859256
  • Target See all TDGF1 ELISA Kits
    TDGF1 (Teratocarcinoma-Derived Growth Factor 1 (TDGF1))
    Binding Specificity
    AA 26-150
    Reactivity
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Mouse
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    62.5-4000 pg/mL
    Minimum Detection Limit
    62.5 pg/mL
    Application
    ELISA
    Purpose
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse Cripto/TDGF1
    Brand
    PicoKine™
    Sample Type
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytical Method
    Quantitative
    Specificity
    NSO, R26-Q150
    Cross-Reactivity (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivity
    <10pg/mL
    Material not included
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: NSO
    Immunogen sequence: R26-Q150
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  • Application Notes
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Comment

    Tissue Specificity: Expressed at low level in specific organs of the adult animal such as spleen, heart, lung and brain. During gastrulation, expressed in the forming mesoderm. In later stages of the developing heart, expression is restricted to the truncus arteriosus.

    Plate
    Pre-coated
    Protocol
    mouse Cripto ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Cripto has been precoated onto 96-well plates. Standards (NSO, R26-Q150) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Cripto is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Cripto amount of sample captured in plate.
    Assay Procedure

    Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse Cripto standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse Cripto standard solution and each sample is measured in duplicate.

    Assay Precision
    • Sample 1: n=16, Mean(pg/ml): 675, Standard deviation: 34.42, CV(%): 5.1
    • Sample 2: n=16, Mean(pg/ml): 1810, Standard deviation: 108.6, CV(%): 6.0
    • Sample 3: n=16, Mean(pg/ml): 2944, Standard deviation: 197.24, CV(%): 6.7,
    • Sample 1: n=24, Mean(pg/ml): 792, Standard deviation: 43.56, CV(%): 5.5
    • Sample 2: n=24, Mean(pg/ml): 1948, Standard deviation: 140.25, CV(%): 7.2
    • Sample 3: n=24, Mean(pg/ml): 3107, Standard deviation: 211.27, CV(%): 6.8
    Restrictions
    For Research Use only
  • Handling Advice
    Avoid multiple freeze-thaw cycles.
    Storage
    -20 °C,4 °C
    Storage Comment
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Expiry Date
    12 months
  • Target See all TDGF1 ELISA Kits
    TDGF1 (Teratocarcinoma-Derived Growth Factor 1 (TDGF1))
    Alternative Name
    TDGF1 (TDGF1 Products)
    Synonyms
    CR ELISA Kit, CRGF ELISA Kit, CRIPTO ELISA Kit, CR1 ELISA Kit, cripto ELISA Kit, cb85 ELISA Kit, tdgf1 ELISA Kit, wu:fe01d04 ELISA Kit, zgc:109829 ELISA Kit, Cripto-1 ELISA Kit, cr1 ELISA Kit, cr1a ELISA Kit, frl-1 ELISA Kit, frl1 ELISA Kit, xcr1 ELISA Kit, TDGF1 ELISA Kit, tdgf3 ELISA Kit, cripto-3 ELISA Kit, teratocarcinoma-derived growth factor 1 ELISA Kit, teratocarcinoma-derived growth factor 1, member 3 L homeolog ELISA Kit, teratocarcinoma-derived growth factor 1, member 1 ELISA Kit, TDGF1 ELISA Kit, Tdgf1 ELISA Kit, tdgf1 ELISA Kit, tdgf1.3.L ELISA Kit, tdgf1.1 ELISA Kit
    Background

    Protein Function: Could play a role in the determination of the epiblastic cells that subsequently give rise to the mesoderm.

    Background: Teratocarcinoma-derived growth factor 1 is a protein that in humans is encoded by the TDGF1 gene. It is mapped to 3p23-p21. The protein is an extracellular, membrane-bound signaling protein that plays an essential role in embryonic developmentand tumor growth. Mutations in this gene are associated with forebrain defects. Pseudogenes of this gene are found on chromosomes 2, 3, 6, 8, 19 and X. Alternate splicing results in multiple transcript variants.

    Synonyms: Teratocarcinoma-derived growth factor,Cripto growth factor,Epidermal growth factor-like Cripto protein,Tdgf1,Cripto,

    Full Gene Name: Teratocarcinoma-derived growth factor

    Cellular Localisation: Cell membrane, Lipid-anchor, GPI-anchor.
    Gene ID
    21667
    UniProt
    P51865
    Pathways
    EGFR Signaling Pathway
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