CXCL2 ELISA Kit

Catalog No. ABIN2859287

Product Details CXCL2 ELISA Kit

Target: CXCL2 (Chemokine (C-X-C Motif) Ligand 2 (CXCL2))

Binding Specificity: AA 32-100

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.6-1000 pg/mL

Minimum Detection Limit: 15.6 pg/mL

Application: ELISA

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat CXCL2/MIP-2

Brand: PicoKine™

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Analytical Method: Quantitative

Specificity: E.coli, S32-N100

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: <10pg/mL

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: E.coli
Immunogen sequence: S32-N100

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Comment:

Tissue Specificity: At least expressed in the lung and trachea.

Plate: Pre-coated

Protocol: rat MIP-2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for MIP-2 has been precoated onto 96-well plates. Standards (E.coli, S32-N100) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MIP-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat MIP-2 amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL rat MIP-2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each rat MIP-2 standard solution and each sample be measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(pg/ml): 113, Standard deviation: 4.97, CV(%): 4.4
• Sample 2: n=16, Mean(pg/ml): 355, Standard deviation: 18.46, CV(%): 5.2
• Sample 3: n=16, Mean(pg/ml): 627, Standard deviation: 25.73, CV(%): 5.7,
• Sample 1: n=24, Mean(pg/ml): 134, Standard deviation: 6.96, CV(%): 5.2
• Sample 2: n=24, Mean(pg/ml): 387, Standard deviation: 21.28, CV(%): 5.5
• Sample 3: n=24, Mean(pg/ml): 653, Standard deviation: 39.18, CV(%): 6.0

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

References for CXCL2 ELISA Kit (ABIN2859287)

Zhu, He, Liu, Zhang, Yang, Liu, Wang: "Alpha 1-antitrypsin ameliorates ventilator-induced lung injury in rats by inhibiting inflammatory responses and apoptosis." in: Experimental biology and medicine (Maywood, N.J.), Vol. 243, Issue 1, pp. 87-95, (2018) (PubMed).

Mei, Wang, Li, Liu, Lu, Li, Zhang, Tian: "Dusuqing granules (DSQ) suppress inflammation in Klebsiella pneumonia rat via NF-κB/MAPK signaling." in: BMC complementary and alternative medicine, Vol. 17, Issue 1, pp. 216, (2017) (PubMed).

Target Details for CXCL2

Target: CXCL2 (Chemokine (C-X-C Motif) Ligand 2 (CXCL2))

Alternative Name: CXCL2 (CXCL2 Products)

Synonyms: CINC-2a ELISA Kit, GRO2 ELISA Kit, GROb ELISA Kit, MGSA-b ELISA Kit, MIP-2a ELISA Kit, MIP2 ELISA Kit, MIP2A ELISA Kit, SCYB2 ELISA Kit, GRO3 ELISA Kit, Gro2 ELISA Kit, MIP-2 ELISA Kit, Mgsa-b ELISA Kit, Mip2 ELISA Kit, Scyb ELISA Kit, Scyb2 ELISA Kit, Mip-2 ELISA Kit, GROB ELISA Kit, MIP-2alpha ELISA Kit, C-X-C motif chemokine ligand 2 ELISA Kit, chemokine (C-X-C motif) ligand 2 ELISA Kit, CXCL2 ELISA Kit, Cxcl2 ELISA Kit

Background:

Protein Function: Chemotactic for human polymorphonuclear leukocytes but does not induce chemokinesis or an oxidative burst. Contributes to neutrophil activation during inflammation.

Background: MIP is a member of the aquaporin family of membrane-bound water channels. MIP family proteins are thought to contain 6 TM domains. Sequence analysis suggests that the proteins may have arisen through tandem, intragenic duplication from an ancestral protein that contained 3 TM domains. Major intrinsic protein (MIP, also called MP26) is the predominant fiber cell membrane protein of the ocular lens. The major intrinsic protein (MIP) of the vertebrate eye lens is the first identified member of a sequence-related family of cell-membrane proteins that appears to have evolved by gene duplication. Several members of the MIP family transport water (aquaporins), glycerol and other small molecules in microbial, plant and animal cells.

Synonyms: C-X-C motif chemokine 2,Cytokine-induced neutrophil chemoattractant 3,CINC-3,Macrophage inflammatory protein 2,MIP2,Cxcl2,Cinc3, Mip-2, Mip2, Scyb2,

Full Gene Name: C-X-C motif chemokine 2

Cellular Localisation: Secreted.

Gene ID: 114105

UniProt: P30348

Pathways: Cellular Response to Molecule of Bacterial Origin