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NBL1 ELISA Kit

NBL1 Reactivity: Human AA 17-181 Colorimetric Sandwich ELISA 125-8000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Catalog No. ABIN2859289
  • Target See all NBL1 ELISA Kits
    NBL1 (Neuroblastoma 1, DAN Family BMP Antagonist (NBL1))
    Binding Specificity
    AA 17-181
    Reactivity
    • 5
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    125-8000 pg/mL
    Minimum Detection Limit
    125 pg/mL
    Application
    ELISA
    Purpose
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human DAN/NBL1
    Brand
    PicoKine™
    Sample Type
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytical Method
    Quantitative
    Specificity
    NSO, A17-D181
    Cross-Reactivity (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivity
    <10pg/mL
    Material not included
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: NSO
    Immunogen sequence: A17-D181
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  • Application Notes
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Comment

    Tissue Specificity: Most abundant in normal lung and meningioma.

    Plate
    Pre-coated
    Protocol
    human DAN ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for DAN has been precoated onto 96-well plates. Standards (NSO, A17-D181) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for DAN is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human DAN amount of sample captured in plate.
    Assay Procedure

    Aliquot 0.1 mL per well of the 8000pg/mL, 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL human DAN standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human DAN standard solution and each sample be measured in duplicate.

    Assay Precision
    • Sample 1: n=16, Mean(pg/ml): 1257, Standard deviation: 72.90, CV(%): 5.8
    • Sample 2: n=16, Mean(pg/ml): 3204, Standard deviation: 189.03, CV(%): 5.9
    • Sample 3: n=16, Mean(pg/ml): 5394, Standard deviation: 334.42, CV(%): 6.2,
    • Sample 1: n=24, Mean(pg/ml): 1020, Standard deviation: 65.28, CV(%): 6.4
    • Sample 2: n=24, Mean(pg/ml): 3562, Standard deviation: 235.09, CV(%): 6.6
    • Sample 3: n=24, Mean(pg/ml): 4975, Standard deviation: 353.22, CV(%): 7.1
    Restrictions
    For Research Use only
  • Handling Advice
    Avoid multiple freeze-thaw cycles.
    Storage
    -20 °C,4 °C
    Storage Comment
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Expiry Date
    12 months
  • Target See all NBL1 ELISA Kits
    NBL1 (Neuroblastoma 1, DAN Family BMP Antagonist (NBL1))
    Alternative Name
    NBL1 (NBL1 Products)
    Synonyms
    D1S1733E ELISA Kit, DAN ELISA Kit, DAND1 ELISA Kit, NB ELISA Kit, NO3 ELISA Kit, D4H1S1733E ELISA Kit, Dana ELISA Kit, dand1 ELISA Kit, nbl1-a ELISA Kit, no3 ELISA Kit, 4.1N ELISA Kit, Epb41l1 ELISA Kit, NBL1 ELISA Kit, mKIAA0338 ELISA Kit, C1orf151-NBL1 ELISA Kit, dan ELISA Kit, zgc:77251 ELISA Kit, neuroblastoma 1, DAN family BMP antagonist ELISA Kit, neuroblastoma, suppression of tumorigenicity 1 ELISA Kit, neuroblastoma 1, DAN family BMP antagonist L homeolog ELISA Kit, neuroblastoma suppressor of tumorigenicity 1 ELISA Kit, erythrocyte membrane protein band 4.1 like 1 ELISA Kit, MINOS1-NBL1 readthrough ELISA Kit, NBL1 ELISA Kit, Nbl1 ELISA Kit, nbl1.L ELISA Kit, LOC535280 ELISA Kit, Epb41l1 ELISA Kit, MINOS1-NBL1 ELISA Kit, nbl1 ELISA Kit
    Background

    Protein Function: Possible candidate as a tumor suppressor gene of neuroblastoma. May play an important role in preventing cells from entering the final stage (G1/S) of the transformation process.

    Background: Differential screening-selected gene aberrative in neuroblastoma (DAN) is a member of the DAN family of secreted glycoproteins that are putative BMP antagonists. The NBL1 gene, also known as DAN, is originally cloned from a normal rat fibroblast cDNA library by a differential screening method. The human DAN gene is mapped to chromosome 1p36.13-p36. It is found that the DAN gene possesses a tumor suppressive activity when overexpressed in v-src transformed cells.

    Synonyms: Neuroblastoma suppressor of tumorigenicity 1,DAN domain family member 1,Protein N03,Zinc finger protein DAN,NBL1,DAN, DAND1,

    Full Gene Name: Neuroblastoma suppressor of tumorigenicity 1

    Cellular Localisation: Secreted.
    Gene ID
    4681
    UniProt
    P41271
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