NBL1 ELISA Kit

Catalog No. ABIN2859290

Product Details NBL1 ELISA Kit

Target: NBL1 (Neuroblastoma 1, DAN Family BMP Antagonist (NBL1))

Binding Specificity: AA 17-178

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 62.5-4000 pg/mL

Minimum Detection Limit: 62.5 pg/mL

Application: ELISA

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse DAN/NBL1

Brand: PicoKine™

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Analytical Method: Quantitative

Specificity: NSO, A17-D178

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: <10pg/mL

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: A17-D178

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Plate: Pre-coated

Protocol: mouse DAN ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for DAN has been precoated onto 96-well plates. Standards (NSO, A17-D178) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for DAN is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse DAN amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse DAN standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse DAN standard solution and each sample is measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(pg/ml): 675, Standard deviation: 37.12, CV(%): 5.5
• Sample 2: n=16, Mean(pg/ml): 1810, Standard deviation: 112.22, CV(%): 6.2
• Sample 3: n=16, Mean(pg/ml): 2944, Standard deviation: 197.24, CV(%): 6.7,
• Sample 1: n=24, Mean(pg/ml): 792, Standard deviation: 50.68, CV(%): 6.4
• Sample 2: n=24, Mean(pg/ml): 1948, Standard deviation: 146.10, CV(%): 7.5
• Sample 3: n=24, Mean(pg/ml): 3107, Standard deviation: 242.34, CV(%): 7.8

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

Target Details for NBL1

Target: NBL1 (Neuroblastoma 1, DAN Family BMP Antagonist (NBL1))

Alternative Name: NBL1 (NBL1 Products)

Synonyms: D1S1733E ELISA Kit, DAN ELISA Kit, DAND1 ELISA Kit, NB ELISA Kit, NO3 ELISA Kit, D4H1S1733E ELISA Kit, Dana ELISA Kit, dand1 ELISA Kit, nbl1-a ELISA Kit, no3 ELISA Kit, 4.1N ELISA Kit, Epb41l1 ELISA Kit, NBL1 ELISA Kit, mKIAA0338 ELISA Kit, C1orf151-NBL1 ELISA Kit, dan ELISA Kit, zgc:77251 ELISA Kit, neuroblastoma 1, DAN family BMP antagonist ELISA Kit, neuroblastoma, suppression of tumorigenicity 1 ELISA Kit, neuroblastoma 1, DAN family BMP antagonist L homeolog ELISA Kit, neuroblastoma suppressor of tumorigenicity 1 ELISA Kit, erythrocyte membrane protein band 4.1 like 1 ELISA Kit, MINOS1-NBL1 readthrough ELISA Kit, NBL1 ELISA Kit, Nbl1 ELISA Kit, nbl1.L ELISA Kit, LOC535280 ELISA Kit, Epb41l1 ELISA Kit, MINOS1-NBL1 ELISA Kit, nbl1 ELISA Kit

Background:

Protein Function: Possible candidate as a tumor suppressor gene of neuroblastoma. May play an important role in preventing cells from entering the final stage (G1/S) of the transformation process.

Background: Differential screening-selected gene aberrative in neuroblastoma (DAN) is a member of the DAN family of secreted glycoproteins that are putative BMP antagonists. The NBL1 gene, also known as DAN, is originally cloned from a normal rat fibroblast cDNA library by a differential screening method. The human DAN gene is mapped to chromosome 1p36.13-p36. It is found that the DAN gene possesses a tumor suppressive activity when overexpressed in v-src transformed cells.

Synonyms: Neuroblastoma suppressor of tumorigenicity 1,N03,Zinc finger protein DAN,Nbl1,Dan, Dana,

Full Gene Name: Neuroblastoma suppressor of tumorigenicity 1

Cellular Localisation: Secreted.

Gene ID: 17965

UniProt: Q61477