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CSF3R ELISA Kit

CSF3R Reactivity: Human AA 25-621 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Catalog No. ABIN2859293
  • Target See all CSF3R ELISA Kits
    CSF3R (Colony Stimulating Factor 3 Receptor (Granulocyte) (CSF3R))
    Binding Specificity
    AA 25-621
    Reactivity
    • 5
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    31.2-2000 pg/mL
    Minimum Detection Limit
    31.2 pg/mL
    Application
    ELISA
    Purpose
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human CSF3R/G-CSF R
    Brand
    PicoKine™
    Sample Type
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytical Method
    Quantitative
    Specificity
    NSO, E25-P621
    Cross-Reactivity (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivity
    <10pg/mL
    Material not included
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Expression system for standard: NSO
    Immunogen sequence: E25-P621
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  • Application Notes
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Comment

    Tissue Specificity: One or several isoforms have been found in myelogenous leukemia cell line KG-1, leukemia U-937 cell line, in bone marrow cells, placenta, and peripheral blood granulocytes. Isoform GCSFR-2 is found only in leukemia U-937 cells. Isoform GCSFR-3 is highly expressed in placenta.

    Plate
    Pre-coated
    Protocol
    human CSF3R ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CSF3R has been precoated onto 96-well plates. Standards(NSO, E25-P621) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CSF3R is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CSF3R amount of sample captured in plate.
    Assay Procedure

    Aliquot 0.1 mL per well of the 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human CSF3R standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum and plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human CSF3R standard solution and each sample be measured in duplicate.

    Assay Precision
    • Sample 1: n=16, Mean(pg/ml): 236, Standard deviation: 12.51, CV(%): 5.3
    • Sample 2: n=16, Mean(pg/ml): 819, Standard deviation: 50, CV(%): 6.1
    • Sample 3: n=16, Mean(pg/ml): 1237, Standard deviation: 59.4, CV(%): 4.8,
    • Sample 1: n=24, Mean(pg/ml): 326, Standard deviation: 21.19, CV(%): 6.5
    • Sample 2: n=24, Mean(pg/ml): 928, Standard deviation: 66.82, CV(%): 7.2
    • Sample 3: n=24, Mean(pg/ml): 1528, Standard deviation: 84.04, CV(%): 5.5
    Restrictions
    For Research Use only
  • Handling Advice
    Avoid multiple freeze-thaw cycles.
    Storage
    -20 °C,4 °C
    Storage Comment
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Expiry Date
    12 months
  • Target See all CSF3R ELISA Kits
    CSF3R (Colony Stimulating Factor 3 Receptor (Granulocyte) (CSF3R))
    Alternative Name
    CSF3R (CSF3R Products)
    Synonyms
    CD114 ELISA Kit, GCSFR ELISA Kit, Cd114 ELISA Kit, Csfgr ELISA Kit, G-CSF-R ELISA Kit, G-CSFR ELISA Kit, CSF3R ELISA Kit, gcsfr ELISA Kit, cd114 ELISA Kit, colony stimulating factor 3 receptor ELISA Kit, colony stimulating factor 3 receptor (granulocyte) ELISA Kit, colony stimulating factor 3 receptor L homeolog ELISA Kit, granulocyte colony-stimulating factor receptor ELISA Kit, CSF3R ELISA Kit, Csf3r ELISA Kit, csf3r ELISA Kit, csf3r.L ELISA Kit, LOC100433338 ELISA Kit
    Background

    Protein Function: Receptor for granulocyte colony-stimulating factor (CSF3), essential for granulocytic maturation. Plays a crucial role in the proliferation, differientation and survival of cells along the neutrophilic lineage. In addition it may function in some adhesion or recognition events at the cell surface. .

    Background: The granulocyte colony-stimulating factor receptor (G-CSF-R), also known as CD114 (Cluster of Differentiation 114), is a protein that in humans is encoded by the CSF3R gene. It is mapped to 1p35-p34.3. G-CSF-R is a cell-surface receptor for the granulocyte colony-stimulating factor (G-CSF). And the G-CSF-R is a transmembrane receptor that consists of an extracellular ligand-binding portion, a transmembrane domain, and the cytoplasmic portion that is responsible for signal transduction. In addition, GCSF-R ligand-binding is associated with dimerization of the receptor and signal transduction through proteins including Jak, Lyn, STAT, andErk1/2.

    Synonyms: Granulocyte colony-stimulating factor receptor,G-CSF receptor,G-CSF-R,CD114,CSF3R,GCSFR,

    Full Gene Name: Granulocyte colony-stimulating factor receptor

    Cellular Localisation: Isoform 2: Secreted.
    Gene ID
    1441
    UniProt
    Q99062
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