ADAMTS13 ELISA Kit

Catalog No. ABIN2859307

Product Details ADAMTS13 ELISA Kit

Target: ADAMTS13 (ADAM Metallopeptidase with Thrombospondin Type 1 Motif, 13 (ADAMTS13))

Binding Specificity: AA 34-688

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.78-50 ng/mL

Minimum Detection Limit: 0.78 ng/mL

Application: ELISA

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human ADAMTS13

Brand: PicoKine™

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate)

Analytical Method: Quantitative

Specificity: CHO, Q34-W688

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: <20pg/mL

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: CHO
Immunogen sequence: Q34-W688

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Comment:

Tissue Specificity: Plasma. Expressed primarily in liver. .

Plate: Pre-coated

Protocol: human ADAMTS13 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for ADAMTS13 has been precoated onto 96-well plates. Standards(CHO, Q34-W688) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ADAMTS13 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human ADAMTS13 amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL, 0.78 ng/mL human ADAMTS13 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each human ADAMTS13 standard solution and each sample is measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(ng/ml): 5.62, Standard deviation: 0.259, CV(%): 4.6
• Sample 2: n=16, Mean(ng/ml): 10.1, Standard deviation: 0.444, CV(%): 4.4
• Sample 3: n=16, Mean(ng/ml): 25.5, Standard deviation: 1.07, CV(%): 4.2,
• Sample 1: n=24, Mean(ng/ml): 6.23, Standard deviation: 0.467, CV(%): 7.5
• Sample 2: n=24, Mean(ng/ml): 12.1, Standard deviation: 0.835, CV(%): 6.9
• Sample 3: n=24, Mean(ng/ml): 26.4, Standard deviation: 1.95, CV(%): 7.4

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

Target Details for ADAMTS13

Target: ADAMTS13 (ADAM Metallopeptidase with Thrombospondin Type 1 Motif, 13 (ADAMTS13))

Alternative Name: ADAMTS13 (ADAMTS13 Products)

Synonyms: ADAMTS13 ELISA Kit, ADAM-TS13 ELISA Kit, ADAMTS-13 ELISA Kit, C9orf8 ELISA Kit, VWFCP ELISA Kit, vWF-CP ELISA Kit, Gm710 ELISA Kit, ADAM metallopeptidase with thrombospondin type 1 motif 13 ELISA Kit, a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 13 ELISA Kit, ADAM metallopeptidase with thrombospondin type 1 motif, 13 ELISA Kit, ADAMTS13 ELISA Kit, Adamts13 ELISA Kit, adamts13 ELISA Kit

Background:

Protein Function: Cleaves the vWF multimers in plasma into smaller forms thereby controlling vWF-mediated platelet thrombus formation. .

Background: ADAMTS13 is a zinc-containing metalloprotease enzyme. The ADAMTS13 gene is mapped to chromosome 9q34 by genomic sequence analysis. It is reported that a metal-containing proteolytic enzyme (metalloprotease) in normal plasma cleaves the peptide bond between tyrosine at position 842 and methionine at position 843 in monomeric subunits of von Willebrand factor, thereby degrading the large multimers. And it is confirmed that the ADAMTS13 gene encodes the von Willebrand factor-cleaving protease (VWFCP). ADAMTS13 is secreted in blood and degrades large vWf multimers, decreasing their activity.

Synonyms: A disintegrin and metalloproteinase with thrombospondin motifs 13,ADAM-TS 13,ADAM-TS13,ADAMTS-13,3.4.24.87,von Willebrand factor-cleaving protease,vWF-CP,vWF-cleaving protease,ADAMTS13,C9orf8,UNQ6102/PRO20085,

Full Gene Name: A disintegrin and metalloproteinase with thrombospondin motifs 13

Cellular Localisation: Secreted . Secretion enhanced by O-fucosylation of TSP type-1 repeats.

Gene ID: 11093

UniProt: Q76LX8