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MethylCollector Ultra Kit Kit

MeDIP, Purif
Catalog No. ABIN2866132
$612.50
Plus shipping costs $45.00
30 tests
local_shipping Shipping to: United States
Delivery in 1 to 2 Business Days
  • Application
    Methylated DNA Immunoprecipitation (MeDIP), Purification (Purif)
    Specificity
    The MethylCollector™ Ultra Kit is Quality Control tested to ensure that the reagents in the kit can be used to enrich for methylated genomic DNA fragments.
  • Comment

    MethylCollector™ Ultra is performed on 100 ng of the MseI digested human, male genomic DNA included in the kit under both high and low salt binding conditions. Enrichment of CpG-methylated DNA is confirmed using real time PCR analysis on the unbound and eluted fractions with the provided NBR2, Xist and GAPDH PCR primer mixes. With high salt binding conditions the control DNA should have at least a 10-fold enrichment of methylated DNA bound and eluted from the protein complex as detected with the NBR2 PCR Primer Mix (Figure 1). This means that of the total DNA recovered, there is ten times as much methylated DNA recovered in the eluted fraction as compared to the unbound fraction for the same locus. With low salt binding conditions the control DNA should have at least a 10-fold enrichment of methylated DNA bound and eluted from the protein complex as detected with the Xist PCR Primer Mix (Figure 2). This means that of the total DNA recovered, there is ten times as much methylated DNA recovered in the eluted fraction as compared to the unbound fraction for the same locus. There should be less than 5% of methylated DNA detected from the eluted fraction with the GAPDH Primer Mix, as this is an unmethylated locus that should only be detected in the unbound fraction. GAPDH, glyceraldehyde-3-phosphate dehydrogenase, is an unmethylated promoter in the control DNA. The provided primer mix amplifies a 69 bp region containing 7 CpGs. Xist, X inactive specific transcript, is a methylated promoter in the control DNA. The provided primer mix amplifies a 178 bp region containing 8 CpGs. NBR2, neighbor of BRCA1 gene 2, is a methylated promoter in the control DNA. The provided primer mix amplifies a 103 bp region containing 7 CpGs.

    Restrictions
    For Research Use only
  • Storage Comment
    The magnetic nickel beads should be stored at 4°C. All other components can be stored at -20°C. See the manual for details. This product is guaranteed stable for 6 months from date of receipt when stored properly.
    Expiry Date
    6 months
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