NFYA ELISA Kit (Nuclear Transcription Factor Y, alpha)

Catalog No. ABIN2866251

Product Details NFYA ELISA Kit

Target:

NFYA

Reactivity: Human

Detection Method: Colorimetric

Method Type: DNA-Binding ELISA

Application: ELISA

Purpose: DNA-binding ELISA that facilitate the study of transcription factor activation in mammalien tissue and cell culture extracts.

Brand: TransAM®

Sample Type: Cell Extracts, Tissue Samples

Analytical Method: Quantitative

Specificity: TransAM NF-YA Kits are tested for sensitivity and specificity in detecting NF-YA in nuclear extracts from Jurkat cells.

Characteristics: Transcription factors are DNA-binding proteins that tightly regulate gene expression. They consist of two distinct domains - one that displays high affinity for a specific DNA sequence and one that confers transcriptional activity. Transcription factors are activated by phosphorylation of specific residues or by processing bound inhibitory proteins. Understanding and quantifying transcription factors is essential for the study of cell functions in relation to differentiation, brain activity, immune response, inflammation and various disease states. TransAM® Kits are sensitive, non-radioactive transcription factor ELISA kits that facilitate the study of transcription factor activation in mammalian tissue and cell extracts.
TransAM® Kits are DNA-binding ELISAs that facilitate the study of transcription factor activation in mammalian tissue and cell extracts. Each kit includes a 96-stripwell plate in which multiple copies of a specific double-stranded oligonucleotide have been immobilized. When nuclear or whole-cell extract is added, activated transcription factor of interest binds the oligonucleotide at its consensus binding site and is quantified using the included antibody, which is specific for the bound, active form of the transcription factor being studied.

Components: One or five 96-well plate(s) with plate sealer(s), primary antibody, HRP-conjugated secondary antibody, wild-type and mutated competitor oligonucleotides, positive control cell extract, DTT, Protease Inhibitor Cocktail, Lysis, Binding, 10X Washing and 10X Antibody Binding Buffers, and Developing and Stop Solutions.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Extract is diluted down to 0.625 μg/well and assayed using the TransAM NF-YA Kit. The assay is performed in the presence or absence of 40 pmol of competitor oligonucleotides containing either a wild-type or mutated form of the NF-YA consensus binding site. The wild-type oligonucleotide prevents NF-YA binding to the probe immobilized on the plate. Conversely, the mutated oligonucleotide has little effect on NF-YA binding (Figure 1). Lot No. 13707002 was developed for 4 minutes.

Assay Time: 5 h

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Except for the cell extract that must be kept at -80°C, the kit components can be stored at -20°C prior to first use. Then, we recommend storing the kit at 4°C except for the oligonucleotides, DTT, Herring Sperm DNA and Protease Inhibitor Cocktail that should be kept at -20°C, and the cell extract at -80°C. This product is guaranteed for 6 months from date of receipt.

Expiry Date: 6 months

Target Details for NFYA

Target:

NFYA

Alternative Name: Nf-Ya (NFYA ELISA Kit Abstract)

Synonyms: wu:fb16e03, ik:tdsubc_2b6, xx:tdsubc_2b6, NFYA, CBF-A, CBF-B, HAP2, NF-YA, AA407810, Cbf-b, SEZ-10, SEZ10, nuclear transcription factor Y, alpha, nuclear transcription factor Y subunit alpha, nuclear transcription factor-Y alpha, nfya, NFYA, Nfya

Pathways: Regulation of Lipid Metabolism by PPARalpha