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Nuclear extracts prepared from unstimulated Jurkat (1 day growth) and U-937 cells are diluted to 0.625 μg/well and assayed using the TransAM c-Myc Kit. The ratio of the signals from the Jurkat cells over the U-937 cells must be above 4. Lot No. 06012008 was developed for 2.5 minutes. It gave a ratio of 403.5 (Figure 1). The endogenous level of c-Myc expression, and this ratio may vary depending on the cell type tested and the treatment used. TransAM c-Myc Kits are also tested for specificity in detecting c-Myc activity. TransAM c-Myc assays are performed in the presence of an excess of oligonucleotide containing a wild-type or mutated c-Myc consensus binding site (Figure 2). At 20X excess, the wild-type oligonucleotide prevents c-Myc binding to the probe immobilized on the plate. Conversely, the mutated oligonucleotide has no effect on c-Myc binding.