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HRV 3C Protease Activity Assay Kit (Colorimetric) Kit

AcA Colorimetric Purified Protein
Catalog No. ABIN2870882
$475.00
Plus shipping costs $45.00
100 tests
local_shipping Shipping to: United States
Delivery in 2 to 3 Business Days
  • Target
    HRV3C Protease
    Detection Method
    Colorimetric
    Application
    Activity Assay (AcA)
    Purpose
    Detection of HRV 3C Protease activity in samples Determine activity of purified HRV 3C Protease
    Sample Type
    Purified Protein
    Characteristics
    HRV 3C Protease Activity assay kit utilizes the ability of a 3C Protease (derived from a HRV rhinovirus-14, EC: 3.4.22.28) to cleave a chromogenic peptide substrate to release a chromophore (pNA) which can be easily quantified using a microplate reader. This assay kit is simple, rapid and can detect HRV 3C Protease activity as low as 50 ng in samples and of purified proteins.
    Detection of HRV 3C Protease activity in samples
    Determine activity of purified HRV 3C Protease
    Components
    HRV 3C Protease Assay Buffer HRV 3C Protease (10 μg, Positive Control) HRV 3C Protease Substrate pNA Standard (0.1 M)
  • Application Notes
    Detection of HRV 3C Protease activity in samples Determine activity of purified HRV 3C Protease
    Comment

    Further details regarding sample type: Purified proteins and cell samples.

    Protocol
    HRV 3C Protease Activity assay kit utilizes the ability of a 3C Protease (derived from a HRV rhinovirus-14, EC: 3.4.22.28) to cleave a chromogenic peptide substrate to release a chromophore (pNA) which can be easily quantified using a microplate reader. This assay kit is simple, rapid and can detect HRV 3C Protease activity as low as 50 ng in samples and of purified proteins.
    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Expiry Date
    12 months
  • Target
    HRV3C Protease
    Alternative Name
    HRV 3C Protease
    Background
    Human rhinovirus (HRV) infections are the most frequent causative agents of common cold and various other upper respiratory tract infections. Rhinoviruses are members of the picornavirus family, which have a positive-sense, single-stranded RNA genome that is translated into a single polyprotein precursor. In the case of HRVs, the viral polyprotein is mainly processed by the proteases (2A and 3C) to generate functional proteins and enzymes.
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