CRH FLISA Kit

Catalog No. ABIN2871101

Product Details CRH FLISA Kit

Target: CRH (Corticotropin Releasing Hormone (CRH))

Reactivity: Mouse, Human, Rat, Dog, Cat

Detection Method: Fluorometric

Method Type: Competition ELISA

Application: ELISA

Analytical Method: Quantitative

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol: The immunoplate in this kit is pre-coated with a secondary antibody and the nonspecific binding sites are blocked. The secondary antibody can bind to the Fc fragment of the primary antibody (peptide antibody) whose Fab fragment will be competitively bound by both biotinylated peptide and peptide standard or targeted peptide in samples. The biotinylated peptide interacts with streptavidin-horseradish peroxidase (SA-HRP) which catalyzes the substrate. The fluorescence intensity is directly proportional to the amount of biotinylated peptide-SA-HRP complex but inversely proportional to the amount of the peptide in standard solutions or samples. This is due to the competitive binding of the biotinylated peptide with the standard peptide or samples to the peptide antibody (primary antibody). A standard curve of known concentration can be established accordingly. The unknown concentration in samples can be determined by extrapolation to this standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Target Details

Target: CRH (Corticotropin Releasing Hormone (CRH))

Alternative Name: Corticotropin Releasing Factor (CRF) (CRH Products)

Synonyms: CRF FLISA Kit, Gm1347 FLISA Kit, crf FLISA Kit, crh FLISA Kit, zgc:101864 FLISA Kit, CRH FLISA Kit, corticoliberin FLISA Kit, crhb FLISA Kit, crh.1-a FLISA Kit, crha FLISA Kit, corticotropin releasing hormone FLISA Kit, corticotropin releasing hormone b FLISA Kit, corticotropin releasing hormone S homeolog FLISA Kit, corticotropin releasing hormone L homeolog FLISA Kit, Crh FLISA Kit, CRH FLISA Kit, crhb FLISA Kit, crh FLISA Kit, crh.S FLISA Kit, crh.L FLISA Kit

Pathways: Positive Regulation of Peptide Hormone Secretion, Hormone Activity, Negative Regulation of Hormone Secretion, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Feeding Behaviour