GNRH1 FLISA Kit

Catalog No. ABIN2871150

Product Details GNRH1 FLISA Kit

Target: GNRH1 (Gonadotropin-Releasing Hormone 1 (Luteinizing-Releasing Hormone) (GNRH1))

Reactivity: Human, Rat, Pig

Detection Method: Fluorometric

Method Type: Competition ELISA

Application: ELISA

Analytical Method: Quantitative

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol: The immunoplate in this kit is pre-coated with a secondary antibody and the nonspecific binding sites are blocked. The secondary antibody can bind to the Fc fragment of the primary antibody (peptide antibody) whose Fab fragment will be competitively bound by both biotinylated peptide and peptide standard or targeted peptide in samples. The biotinylated peptide interacts with streptavidin-horseradish peroxidase (SA-HRP) which catalyzes the substrate. The fluorescence intensity is directly proportional to the amount of biotinylated peptide-SA-HRP complex but inversely proportional to the amount of the peptide in standard solutions or samples. This is due to the competitive binding of the biotinylated peptide with the standard peptide or samples to the peptide antibody (primary antibody). A standard curve of known concentration can be established accordingly. The unknown concentration in samples can be determined by extrapolation to this standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Target Details

Target: GNRH1 (Gonadotropin-Releasing Hormone 1 (Luteinizing-Releasing Hormone) (GNRH1))

Alternative Name: Luteinizing Hormone-Releasing Hormone (LH-RH) (GNRH1 Products)

Synonyms: GNRH FLISA Kit, GRH FLISA Kit, HH12 FLISA Kit, LHRH FLISA Kit, LNRH FLISA Kit, Gnrh FLISA Kit, Gnrh2 FLISA Kit, Lhrh1 FLISA Kit, Lnrh FLISA Kit, hpg FLISA Kit, Gnrha FLISA Kit, Lhrh FLISA Kit, Rgnrhg1 FLISA Kit, CGNRH-I FLISA Kit, GnRH-I FLISA Kit, GnRH FLISA Kit, gnrh FLISA Kit, gnrh1 FLISA Kit, grh FLISA Kit, lhrh FLISA Kit, lnrh FLISA Kit, mdGnRH FLISA Kit, mdgnrh FLISA Kit, mfGnRH FLISA Kit, gonadotropin releasing hormone 1 FLISA Kit, gonadotropin releasing hormone 1 L homeolog FLISA Kit, prepro-gonadotropin-releasing hormone FLISA Kit, GNRH1 FLISA Kit, Gnrh1 FLISA Kit, gnrh1.L FLISA Kit, gnrh1 FLISA Kit

Pathways: Hormone Activity