MSH alpha FLISA Kit
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- Target See all MSH alpha FLISA Kits
- MSH alpha
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Reactivity
- Human, Mouse, Rat
- Detection Method
- Fluorometric
- Method Type
- Competition ELISA
- Application
- ELISA
- Analytical Method
- Quantitative
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Plate
- Pre-coated
- Protocol
- The immunoplate in this kit is pre-coated with a secondary antibody and the nonspecific binding sites are blocked. The secondary antibody can bind to the Fc fragment of the primary antibody (peptide antibody) whose Fab fragment will be competitively bound by both biotinylated peptide and peptide standard or targeted peptide in samples. The biotinylated peptide interacts with streptavidin-horseradish peroxidase (SA-HRP) which catalyzes the substrate. The fluorescence intensity is directly proportional to the amount of biotinylated peptide-SA-HRP complex but inversely proportional to the amount of the peptide in standard solutions or samples. This is due to the competitive binding of the biotinylated peptide with the standard peptide or samples to the peptide antibody (primary antibody). A standard curve of known concentration can be established accordingly. The unknown concentration in samples can be determined by extrapolation to this standard curve.
- Restrictions
- For Research Use only
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- Storage
- 4 °C
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- Target See all MSH alpha FLISA Kits
- MSH alpha
- Alternative Name
- Melanocyte Stimulating Hormone alpha (Alpha MSH) (MSH alpha Products)
- Synonyms
- ACTH FLISA Kit, CLIP FLISA Kit, LPH FLISA Kit, MSH FLISA Kit, NPP FLISA Kit, POC FLISA Kit, AMSH FLISA Kit, BE FLISA Kit, Beta-LPH FLISA Kit, Clip FLISA Kit, Gamma-LPH FLISA Kit, Npp FLISA Kit, Pomc-1 FLISA Kit, Pomc1 FLISA Kit, alpha-MSH FLISA Kit, alphaMSH FLISA Kit, beta-MSH FLISA Kit, gamma-MSH FLISA Kit, Pomc2 FLISA Kit, proopiomelanocortin FLISA Kit, STAM binding protein FLISA Kit, pro-opiomelanocortin-alpha FLISA Kit, POMC FLISA Kit, STAMBP FLISA Kit, Pomc FLISA Kit
- Pathways
- Metabolism of Steroid Hormones and Vitamin D, Peptide Hormone Metabolism, Hormone Activity
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