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IDS ELISA Kit

IDS Reactivity: Human AA 26-550 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Catalog No. ABIN3044712
  • Target See all IDS ELISA Kits
    IDS (Iduronate 2-Sulfatase (IDS))
    Binding Specificity
    AA 26-550
    Reactivity
    • 7
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    156-10000 pg/mL
    Minimum Detection Limit
    156 pg/mL
    Application
    ELISA
    Purpose
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IDS
    Brand
    PicoKine™
    Sample Type
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytical Method
    Quantitative
    Specificity
    Expression system for standard: NSO
    Immunogen sequence: S26-P550
    Cross-Reactivity (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensitivity
    <15pg/mL
    Material not included
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogen
    Immunogen sequence: S26-P550
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  • Application Notes
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Comment

    Tissue Specificity: Liver, kidney, lung, and placenta.

    Plate
    Pre-coated
    Protocol
    human IDS ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IDS has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: S26-P550) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IDS is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IDS amount of sample captured in plate.
    Assay Procedure

    Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human IDS standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human IDS standard solution and each sample be measured in duplicate.

    Restrictions
    For Research Use only
  • Buffer
    heparin or EDTA
    Handling Advice
    Avoid multiple freeze-thaw cycles.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Expiry Date
    12 months
  • Target See all IDS ELISA Kits
    IDS (Iduronate 2-Sulfatase (IDS))
    Alternative Name
    IDS (IDS Products)
    Synonyms
    mps2 ELISA Kit, sids ELISA Kit, zgc:158245 ELISA Kit, MPS2 ELISA Kit, SIDS ELISA Kit, AW214631 ELISA Kit, iduronate 2-sulfatase ELISA Kit, IDS ELISA Kit, CpipJ_CPIJ004938 ELISA Kit, ids ELISA Kit, Ids ELISA Kit
    Background

    Protein Function: Required for the lysosomal degradation of heparan sulfate and dermatan sulfate.

    Background: Iduronate 2-sulfatase (IDS) is a sulfatase enzyme associated with Hunter syndrome. It encodes a member of the sulfatase family of proteins. Iduronate 2-sulfatase is involved in the lysosomal degradation of the glycosaminoglycans heparan sulfate and dermatan sulfate. The encoded preproprotein is proteolytically processed to generate two polypeptide chains. Mutations in this gene are associated with the X-linked lysosomal storage disease mucopolysaccharidosis type II, also known as Hunter syndrome. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed.

    Synonyms: Iduronate 2-sulfatase,3.1.6.13,Alpha-L-iduronate sulfate sulfatase,Idursulfase,Iduronate 2-sulfatase 42 kDa chain,Iduronate 2-sulfatase 14 kDa chain,IDS,SIDS,

    Full Gene Name: Iduronate 2-sulfatase

    Cellular Localisation: Lysosome.
    Gene ID
    3423
    UniProt
    P22304
    Pathways
    Glycosaminoglycan Metabolic Process
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