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5mC Antibody with Positive Primer Set Kit

MeDIP Reactivity: Human
Catalog No. ABIN3071710
$527.39
Plus shipping costs $45.00
50 μL
local_shipping Shipping to: United States
Delivery in 7 to 8 Business Days
  • Target
    Reactivity
    Human
    Host
    Mouse
    Clonality
    Monoclonal
    Application
    Methylated DNA Immunoprecipitation (MeDIP)
    Purpose
    MeDIP Validated 5mC Antibody and Positive Primer Set provides a complete set of tools to assist with a successful ChIP assay.
    Characteristics
    5mC (5---Methylcytosine) is a region of DNA that has been epigenetically modified through the addition of a methyl group at the 5th carbon position of cytosine. DNA methylation is a repressive histone modification that is associated with switching off gene expression. This methylation generally occurs at CpG islands and is also associated with genomic imprinting. Aberrant DNA methylation is linked to disease iincluding many cancers.
    Components
    5mC antibody, control mouse IgG, and a positive primer set. Antibody Concentration: 2 mg/mL
  • Application Notes
    5mC 2:1 (chromatin: antibody)
    Mouse IgG 2:1 (chromatin: antibody)
    Positive Primer Set Dilute from 4μM (provided) to 1μM working concentration
    Note: Optimal dilutions should be determined by the user. These volumes are stated as guidelines only.
    Comment

    The MeDIP Validated 5mC Antibody and Positive Primer Set is not suitable for use with non-human species. Antibody is Protein G affinity purified.

    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    Avoid multiple freeze/thaw cycles as this may denature the antibody and degrade the primer sets
  • Target Name (Antigen)
    Background
    5mC (5---Methylcytosine) is a region of DNA that has been epigenetically modified through the addition of a methyl group at the 5th carbon position of cytosine. DNA methylation is a repressive histone modification that is associated with switching off gene expression. This methylation generally occurs at CpG islands and is also associated with genomic imprinting. Aberrant DNA methylation is linked to disease i many cancers.
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