5mC Antibody with Positive Primer Set

Catalog No. ABIN3071710

Product Details

Target:

5mC

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Application: Methylated DNA Immunoprecipitation (MeDIP)

Purpose: MeDIP Validated 5mC Antibody and Positive Primer Set provides a complete set of tools to assist with a successful ChIP assay.

Characteristics: 5mC (5---Methylcytosine) is a region of DNA that has been epigenetically modified through the addition of a methyl group at the 5th carbon position of cytosine. DNA methylation is a repressive histone modification that is associated with switching off gene expression. This methylation generally occurs at CpG islands and is also associated with genomic imprinting. Aberrant DNA methylation is linked to disease iincluding many cancers.

Components: 5mC antibody, control mouse IgG, and a positive primer set. Antibody Concentration: 2 mg/mL

Application Details

Application Notes: 5mC 2:1 (chromatin: antibody)
Mouse IgG 2:1 (chromatin: antibody)
Positive Primer Set Dilute from 4μM (provided) to 1μM working concentration
Note: Optimal dilutions should be determined by the user. These volumes are stated as guidelines only.

Comment:

The MeDIP Validated 5mC Antibody and Positive Primer Set is not suitable for use with non-human species. Antibody is Protein G affinity purified.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: Avoid multiple freeze/thaw cycles as this may denature the antibody and degrade the primer sets

Target Details

Target Name (Antigen):

5mC

Background: 5mC (5---Methylcytosine) is a region of DNA that has been epigenetically modified through the addition of a methyl group at the 5th carbon position of cytosine. DNA methylation is a repressive histone modification that is associated with switching off gene expression. This methylation generally occurs at CpG islands and is also associated with genomic imprinting. Aberrant DNA methylation is linked to disease i many cancers.